{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T14:02:17Z","timestamp":1700316137954},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":5679,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A number of androgen effects on epithelial cells may be mediated by androgen\u2010regulated paracrine factors produced by underlying mesenchymal cells. In previous studies we demonstrated that prostatic stromal cells and testicular peritubular cells, derived from immature rats, produce mediators of androgen action with identical effects on Sertoli cells. In the present paper we further compared the morphological and functional characteristics of both mesenchymal cell types. Cultured prostatic stromal cells and testicular peritubular cells look identical under phase\u2010contrast microscopy, share the ability to form tubular structures and \u201eballs\u201d\ufe01 when cocultured with Sertoli cells, and contain proteins immunoreactive with an antiserum against \u03b1\u2010smooth muscle isoactin. Two\u2010dimensional gel electrophoresis shows that the pattern of proteins produced by both cell types is nearly identical. Conditioned media from stromal and peritubular cells contain a factor that stimulates transferrin and cGMP production in Sertoli cells. The behavior of the active principle in the media from both cell types is comparable. On reverse\u2010phase HPLC the elution profile of this factor is comparable for media from both cell types. In conclusion, these data point to a striking similarity in the morphological and functional characteristics of mesenchymal cells cultured from the prostate and testis.<\/jats:p>","DOI":"10.1002\/pros.2990190203","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T02:08:46Z","timestamp":1172023726000},"page":"99-112","source":"Crossref","is-referenced-by-count":8,"title":["Morphological and functional similarities between cultured prostatic stromal cells and testicular peritubular myoid cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Kristien","family":"Swinnen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludo","family":"Deboel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Cailleau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Heyns","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Verhoeven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"279","article-title":"Mechanisms of organogenetic tissue interaction","volume":"26","author":"Grobstein C","year":"1967","journal-title":"Natl Cancer Inst Monogr"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60088-1"},{"key":"e_1_2_1_4_2","first-page":"559","article-title":"Hormone\u2010induced morphogenesis and growth: role of mesenchymal\u2010epithelial interactions","volume":"39","author":"Cunha GR","year":"1983","journal-title":"Recent Prog Horm Res"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-121-6-2153"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-8-3-338"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(85)90165-0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.1.114"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1016\/S0021-9258(18)69151-3","article-title":"Purification of a paracrine factor, P\u2010Mod\u2010S, produced by testicular peritubular cells that modulates Sertoli cell function","volume":"263","author":"Skinner MK","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(89)90124-X"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-1-142"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-4-1541"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-4-2100"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_16_2","first-page":"44","volume-title":"Cell Biology of the Testis","author":"Mather J","year":"1982"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90114-4"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod42.2.351"},{"key":"e_1_2_1_19_2","unstructured":"FariniD StefaniniM PalombiF:Late expression of smooth muscle specific actin in developing myoid cells. 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