{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:52:48Z","timestamp":1761540768301},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":4217,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In an endeavor to identify marker(s) for prostatic cancer, proteins in prostatic fluids were analyzed by two\u2010dimensional (2\u2010D) gel electrophoresis. The fluids were obtained from five males who had no prostate lesions and five patients each with benign prostatic hyperplasia (BPH) and prostatic carcinoma (PCA). The specimens were collected directly over a mixture of protease inhibitors and centrifuged, and the supernatants were lyophilized and solubilized in sodium dodecyl sulfate mix. Identical amounts of proteins were pooled according to donors' prostate disease and the resulting samples were subjected to 2\u2010D gel analysis employing the ISO\u2010DALT system. The electrophoretograms were developed by silver or double stain.<\/jats:p><jats:p>The samples of each group exhibited distinctive profiles with the exception of similar relative positions of major protein spots. A predominant protein occurring as several charge variants was consistently present in prostatic fluids of patients with PCA. This protein appeared to be a previously unknown constituent that we have called protein D (molecular weight \u223c\ufe0122 kDa and isoelectric point \u223c\ufe014), and was undetectable in the fluids of \u201cnormal\u201d men and patients with BPH. An analysis of pooled, unprocessed urine from PCA patients revealed that perhaps this protein is excreted in urine in very low quantities.<\/jats:p><jats:p>These results strongly suggest that the potential of this protein as a marker for prostatic cancer should be further explored.<\/jats:p>","DOI":"10.1002\/pros.2990260104","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T03:22:56Z","timestamp":1172028176000},"page":"12-18","source":"Crossref","is-referenced-by-count":14,"title":["Analysis of prostatic fluid: Evidence for the presence of a prospective marker for prostatic cancer"],"prefix":"10.1002","volume":"26","author":[{"given":"Phulwinder K.","family":"Grover","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin I.","family":"Resnick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.40.1.9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/15287397909529754"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990030106"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1093\/clinchem\/30.12.2026","article-title":"Systematic characterization of human prostatic fluid proteins with two\u2010dimensional electrophoresis","volume":"30","author":"Tsai YC","year":"1984","journal-title":"Clin Chem"},{"key":"e_1_2_1_6_2","first-page":"1766","article-title":"Two\u2010dimensional electrophoretic analysis of human protatic fluid proteins","volume":"45","author":"Guevara J","year":"1985","journal-title":"Cancer 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