{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T22:24:16Z","timestamp":1772144656740,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Specific and sensitive minimally\u2010invasive biomarker(s) for early detection of prostate cancer is urgently needed to reduce false detection and odds of overtreatment. MicroRNAs (miRNAs) are 19\u201324\u2010nt noncoding RNAs that regulate gene expression, have emerged as promising blood\u2010based biomarkers due to their frequent dysregulation in cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>miRNA levels were quantified in a total of 86 human plasma samples, consisting of 44 prostate cancer patients and 42 individuals with no cancer, using quantitative real\u2010time PCR. Serum PSA and plasma GSTP1 levels were detected by using ELISA assay. The sensitivity and specificity of these miRNAs were evaluated through ROC (Receiver Operating Characteristic) curve analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>To identify sensitive and specific miRNA biomarkers for prostate cancer, target prediction analysis was performed. This analysis highlighted hsa\u2010miR\u2010133a\u20103p, hsa\u2010miR\u2010144\u20103p, hsa\u2010miR\u2010153\u20103p, and hsa\u2010miR\u2010590, which regulate glutathione S\u2010transferase P1 (GSTP1), a gene epigenetically silenced in all stages of prostate cancer. The absolute expression levels of these miRNAs were quantified in plasma samples from 44 prostate cancer patients and 42 noncancer individuals. Sensitivity and specificity were assessed using ROC curve analysis. Binary logistic regression ROC analysis revealed that hsa\u2010miR\u2010133a\u20103p and hsa\u2010miR\u2010153\u20103p demonstrated exceptional specificity and sensitivity for prostate cancer detection, with <jats:italic>p<\/jats:italic> values of &lt;\u20090.0001 and 0.0003, outperforming PSA levels. Further validation in an independent data set of 64 noncancer individuals and 26 prostate cancer patients confirmed significant differences in hsa\u2010miR\u2010133a\u20103p and hsa\u2010miR\u2010153\u20103p expression between the groups. The two\u2010miRNA panel exhibited high diagnostic accuracy, with an area under the curve between 0.98 and 1.0.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that the two\u2010miRNA panel represents a significant advancement over PSA testing and shows strong potential as a reliable blood\u2010based diagnostic tool for prostate cancer detection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.70011","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T05:59:51Z","timestamp":1752127191000},"page":"1235-1244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["miRNAs Regulating GSTP1 as Potential Diagnostic Biomarkers in Prostate Cancer"],"prefix":"10.1002","volume":"85","author":[{"given":"Shiv","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Urology Case Western Reserve University Cleveland Ohio USA"},{"name":"The 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