{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T14:49:47Z","timestamp":1778338187902,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the value of circulating free DNA (cfDNA) in prostate cancer (PCa) by cfDNA assay and analysis of plasma and urinary cfDNA fragmentation to determine the usefulness of this parameter for risk staging and tumor progression monitoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A prospective, longitudinal study was conducted with 143 individuals, including a control group and a cohort of patients with PCa at different stages: localized, metastatic hormone\u2010sensitive (mHSPC), and metastatic castration\u2010resistant (mCRPC). Plasma and urine samples were collected to measure the concentration, fluorescence units (FU), and cfDNA fragmentation, correlating them with clinical and pathological variables.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Plasma cfDNA levels were higher in patients with PCa than in control subjects (14.3\u2009ng\/mL vs. 4.2\u2009ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.04) and even higher in metastatic disease than in localized (20.8\u2009ng\/mL vs. 3.6\u2009ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). The fragmentation size of plasma cfDNA was smaller in metastatic PCa (168.7 base pairs) than in localized PCa (172.8 base pairs,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), suggesting that shorter fragments are associated with more aggressive disease. Following systemic treatment, the patients decreased cfDNA levels (8.3\u2009ng\/mL vs. 4.9\u2009ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.027) and plasma FU (35.2 vs. 12.9,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). In urine, differences were only observed in patients who progressed to CRPC than in those who remained HSPC (261.8\u2009ng\/mL vs. 43.5\u2009ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.046).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The assay and analysis of plasma and urinary cfDNA fragmentation may provide useful biomarkers for PCa diagnosis and follow\u2010up, particularly when differentiating between localized and metastatic disease. These findings are promising, but further research is required to determine their potential utility in clinical risk stratification and treatment monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.70090","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T11:13:19Z","timestamp":1762773199000},"page":"338-348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic and Prognostic Value of cfDNA Concentration and Fragmentation in Prostate Cancer"],"prefix":"10.1002","volume":"86","author":[{"given":"Marta Victoria","family":"Lorenzo\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Miguel","family":"Gim\u00e9nez\u2010Bachs","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Granada","family":"Picazo\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Experimental Research Unit Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Donate\u2010Moreno","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Mart\u00ednez\u2010S\u00e1nchiz","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Amalia","family":"T\u00e1rraga\u2010Honrubia","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonsoles","family":"Navarro\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d3scar","family":"Legido\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Urology General Hospital of Hell\u00edn Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1837-6981","authenticated-orcid":false,"given":"Antonio Santiago","family":"Salinas\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Urology Hospital and University Complex of Albacete Albacete Spain"},{"name":"School of Medicine of Albacete Universidad de Castilla la Mancha Albacete Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2023.04.021"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/almed-2019-0027"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/almed-2023-0034"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2019.100087"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01505-z"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00837-3"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2023.08.010"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2020.07.001"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1272-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108247"},{"key":"e_1_2_11_12_1","first-page":"2318","article-title":"Clinical Applications of Urinary Cell\u2010Free DNA in Cancer: Current Insights and Promising Future","volume":"7","author":"Lu T.","year":"2017","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.02.013"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctarc.2020.100193"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.599602"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw3616"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aat4921"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1677-2"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24943"},{"key":"e_1_2_11_20_1","unstructured":"P.Cornford TilkiD R. C. N.vandenBergh E.Briers D.Eberli et al. Patient Advocate (European Prostate Cancer Coalition\/Europe UOMO). EAU\u2010EANM\u2010ESTRO\u2010ESUR\u2010ISUP\u2010SIOG Guidelines on Prostate Cancer\u20102024 EAU Guidelines. Ed. presented at the EAU Annual Congress Paris April2024. 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