{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:41:06Z","timestamp":1792694466949,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The Hippo pathway effector YAP1 has been implicated in prostate cancer progression, migration, and castration resistance, yet how pharmacological YAP1 inhibition reshapes the prostate cancer proteome remains uncharacterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used label\u2010free quantitative proteomics together with functional assays to characterize the proteomic and phenotypic effects of CA3, a selective YAP1 inhibitor, in androgen\u2010independent PC3 and androgen\u2010sensitive LNCaP prostate cancer cells, and validated key findings with wound\u2010healing, western blot, and immunodetection assays for focal adhesion kinase (FAK), vimentin, occludin, and junctional adhesion molecule A (JAM\u2010A).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CA3 reduced cell viability and YAP1 protein levels in both cell lines in a dose\u2010 and time\u2010dependent manner (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Nonlinear regression analysis yielded 24\u2009h IC50 values of 1.59\u2009\u00b5M (95% CI, 1.38\u20131.83) for PC3 and 0.63\u2009\u00b5M (95% CI, 0.53\u20130.77) for LNCaP, with corresponding 48\u2009h values of 0.59\u2009\u00b5M (95% CI, 0.50\u20130.69) and 0.49\u2009\u00b5M (95% CI, 0.39\u20130.62). CA3 showed markedly lower potency in the non\u2010tumorigenic PNT1A prostate epithelial cell line (IC50 18.87\u2009\u00b5M at 24\u2009h; 4.47\u2009\u00b5M at 48\u2009h), corresponding to an approximately 7\u2010 to 30\u2010fold selectivity window favoring the cancer cell lines. Label\u2010free quantitative proteomics identified 365 and 153 differentially expressed proteins (DEPs) in PC3 and LNCaP cells, respectively. Functional enrichment analysis revealed cell line\u2010dependent alterations in pathways related to RNA metabolism and translation, alongside migration\u2010 and adhesion\u2010associated processes, including focal adhesion, cytoskeletal organization, and guidance signaling. Comparison of these proteins with the Gene Ontology cell migration gene set (GO:0016477) identified EGFR, SRC, TGFB1, IL6, and SCARB1 as hub proteins in PC3 cells, and RAC1, RHOC, NF1, FSCN1, and ASPM in LNCaP cells. Functional validation confirmed that CA3 reduced wound closure in both cell lines, decreased FAK protein levels, selectively reduced vimentin in PC3 cells, increased occludin in both cell lines, and increased JAM\u2010A specifically in PC3 cells (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To our knowledge, this is the first study to characterize the CA3\u2010induced proteome in prostate cancer cells and link these changes to migration\u2010associated protein networks, in cell lines with differing androgen dependence.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.70271","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:34:56Z","timestamp":1792694096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hippo Pathway Modulation Alters the Proteome and Migratory Phenotype of Prostate Cancer Cells"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2444-4258","authenticated-orcid":false,"given":"Merve Gulsen","family":"Bal Albayrak","sequence":"first","affiliation":[{"name":"Department of Molecular Gastroenterology and Hepatology, Gastroenterology and Hepatology Institute Kocaeli University Kocaeli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4902-4022","authenticated-orcid":false,"given":"Tu\u011fcan","family":"Korak","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine 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