{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:26:21Z","timestamp":1792189581894,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,2]],"date-time":"2004-04-02T00:00:00Z","timestamp":1080864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The \u03b3\u2010KTx\u2013type scorpion toxins specific for K<jats:sup>+<\/jats:sup> channels were found to interact with <jats:italic>ERG<\/jats:italic> channels on the turret region, while \u03b1\u2010KTx3.2 Agitoxin\u20102 binds to the pore region of the <jats:italic>Shaker<\/jats:italic> K<jats:sup>+<\/jats:sup> channel, and \u03b1\u2010KTx5.3 BmP05 binds to the intermediate region of the small\u2010conductance calcium\u2010activated K\u2010channel (SK<jats:sub>Ca<\/jats:sub>). In order to explore the critical residues for \u03b3\u2010KTx binding, we determined the NMR structure of native \u03b3\u2010KTx1.1 (CnErg1), a 42 amino acid residues scorpion toxin isolated from the venom of the Mexican scorpion <jats:italic>Centruro\u00efdes noxius<\/jats:italic> Hoffmann, and we used computational evolutionary trace (ET) analysis to predict possible structural and functional features of interacting surfaces. The <jats:sup>1<\/jats:sup>H\u2010NMR three\u2010dimensional solution structure of native ergtoxin (CnErg1) was solved using a total of 452 distance constraints, 13 <jats:sup>3<\/jats:sup>J<jats:sub>NH\u2010H\u03b1<\/jats:sub> and 10 hydrogen bonds. The structure is characterized by 2 segments of \u03b1\u2010helices and a triple\u2010stranded antiparallel \u03b2\u2010sheet stabilized by 4 disulfide bridges. The ET and structural analysis provided indication of the presence of two important amino acid residue clusters, one hydrophobic and the other hydrophilic, that should be involved in the surface contact between the toxin and the channel. Some features of the proposed interacting surface are discussed. Proteins 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.20102","type":"journal-article","created":{"date-parts":[[2004,4,2]],"date-time":"2004-04-02T21:01:20Z","timestamp":1080939680000},"page":"367-375","source":"Crossref","is-referenced-by-count":26,"title":["Exploring structural features of the interaction between the scorpion toxinCnErg1 and <i>ERG<\/i> K<sup>+<\/sup> channels"],"prefix":"10.1002","volume":"56","author":[{"given":"Karine","family":"Fr\u00e9nal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Qi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Wolff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Wecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina 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