{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:56:07Z","timestamp":1783468567689,"version":"3.55.0"},"reference-count":60,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A natural K<jats:sup>+<\/jats:sup> channel blocker, BmKK2 (a member of scorpion toxin subfamily \u03b1\u2010KTx 14), which is composed of 31 amino acid residues and purified from the venom of the Chinese scorpion <jats:italic>Buthus<\/jats:italic> <jats:italic>martensi<\/jats:italic> Karsch, was characterized using whole\u2010cell patch\u2010clamp recording in rat hippocampal neurons. The three dimensional structure of BmKK2 was determined with two\u2010dimensional NMR spectroscopy and molecular modelling techniques. In solution this toxin adopted a common \u03b1\/\u03b2\u2010motif, but showed distinct local conformation in the loop between \u03b1\u2010helix and \u03b2\u2010sheet in comparison with typical short\u2010chain scorpion toxins (e.g., CTX and NTX). Also, the \u03b1 helix is shorter and the \u03b2\u2010sheet element is smaller (each strand consisted only two residues). The unusual structural feature of BmKK2 was attributed to the shorter loop between the \u03b1\u2010helix and \u03b2\u2010sheet and the presence of two consecutive Pro residues at position 21 and 22 in the loop. Moreover, two models of BmKK2\/hKv1.3 channel and BmKK2\/rSK2 channel complexes were simulated with docking calculations. The results demonstrated the existence of a \u03b1\u2010mode binding between the toxin and the channels. The model of BmKK2\/rSK2 channel complex exhibited favorable contacts both in electrostatic and hydrophobic, including a network of five hydrogen bonds and bigger interface containing seven pairs of inter\u2010residue interactions. In contrast, the model of BmKK2\/hKv1.3 channel complex, containing only three pairs of inter\u2010residue interactions, exhibited poor contacts and smaller interface. The results well explained its lower activity towards Kv channel, and predicted that it may prefer a type of SK channel with a narrower entryway as its specific receptor. Proteins 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.20117","type":"journal-article","created":{"date-parts":[[2004,4,2]],"date-time":"2004-04-02T00:01:00Z","timestamp":1080864060000},"page":"835-845","source":"Crossref","is-referenced-by-count":13,"title":["Solution structure of BmKK2, a new potassium channel blocker from the venom of chinese scorpion <i>Buthus martensi<\/i> Karsch"],"prefix":"10.1002","volume":"55","author":[{"given":"Naixia","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuefeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houming","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,4]]},"reference":[{"key":"e_1_2_6_2_2","series-title":"Advances 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