{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T21:35:39Z","timestamp":1769722539083,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T00:00:00Z","timestamp":1100822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This article reports the solution structure of BmTx3B (\u03b1\u2010KTx16.2), a potassium channel blocker belonging to the subfamily \u03b1\u2010KTx16, purified from the venom of the Chinese scorpion <jats:italic>Buthus martensi<\/jats:italic> Karsch. In solution, BmTx3B assumes a typical CS\u03b1\u03b2 motif, with an \u03b1\u2010helix connected to a triple\u2010stranded \u03b2\u2010sheet by 3 disulfide bridges, which belongs to the first structural group of short\u2010chain scorpion toxins. On the other hand, BmTx3B is quite different from other toxins (such as ChTx and AgTx2) of this group in terms of the electrostatic and hydrophobic surface distribution. The functional surface (\u03b2\u2010face) of the molecule is characterized by less basic residues (only 2: Lys28 and Arg35) and extra aromatic residues (Phe1, Phe9, Trp15, and Tyr37). The peptide shows a great preference for the Kca1.1 channel over the Kv channel (about a 10<jats:sup>3<\/jats:sup>\u2010fold difference). The model of BmTx3B\/Kca1.1 channel complex generated by docking and dynamic simulation reveals that the stable binding between the BmTx3B and Kca1.1 channel is favored by a number of aromatic \u03c0\u2013\u03c0 stacking interactions. The influences of these structural features on the kinetic behavior of the toxin binding to Kca1.1 channel are also discussed. Proteins 2005. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.20322","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T19:17:43Z","timestamp":1100891863000},"page":"489-497","source":"Crossref","is-referenced-by-count":13,"title":["The solution structure of BmTx3B, a member of the scorpion toxin subfamily \u03b1\u2010KTx 16"],"prefix":"10.1002","volume":"58","author":[{"given":"Yuefeng","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naixia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houming","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00625.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3150977"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(99)01398-X"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(02)00142-3"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-9639(02)00458-2"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi035412"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(03)00080-4"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(03)00223-2"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030977"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.03.022"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031860"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0473"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac199971061157"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9809371"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(20000101)38:1<70::AID-PROT8>3.0.CO;2-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3435"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi000860s"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20117"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(97)90303-2"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90415-4"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.10.3329"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38560-6"},{"key":"e_1_2_6_24_2","first-page":"1016","article-title":"BmTx3B, 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