{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:42:46Z","timestamp":1791222166211,"version":"4.1.0"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T00:00:00Z","timestamp":1123027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nuclear autoantigenic sperm protein (NASP) is a linker histone binding protein that is cell\u2010cycle regulated. Synchronized HeLa cells are delayed in progression through the G1\/S border when transiently transfected to overexpress full\u2010length NASP, but not the histone\u2010binding site (HBS) deletion mutant (NASP\u2013\u0394HBS). The purpose of the current study was to identify possible NASP\u2010associated proteins in HeLa cell nuclei that could elucidate NASP's influence on the cell cycle and chromatin remodeling. For this purpose, we employed a new approach: mass spectrometry identification of initially cross\u2010linked proteins after their separation in a second dimension by reducing SDS\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE). Of the twelve proteins identified, three appear to be relevant to NASP's function: heat shock protein 90 (HSP90), DNA\u2010activated protein kinase, and ATP\u2010dependent DNA helicase II (70\u2010kDa subunit). Individual protein\u2013protein interactions were tested by immunoprecipitation techniques. This new method can be used for expedited identification of binding partners of different proteins in enriched fractions and as a complementary or alternative strategy to the yeast two\u2010hybrid system and immunoprecipitation methods. Proteins 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.20600","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T21:49:23Z","timestamp":1123105763000},"page":"1-5","source":"Crossref","is-referenced-by-count":17,"title":["Mass spectrometry identification of NASP binding partners in HeLa cells"],"prefix":"10.1002","volume":"61","author":[{"given":"Oleg M.","family":"Alekseev","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard T.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshall R.","family":"Pope","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"O'Rand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,8,3]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.437"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003781200"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210352200"},{"key":"e_1_2_5_5_2","first-page":"43.7","volume-title":"Cells: A Laboratory Manual, 1","author":"Spector DL","year":"1998"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9702344"},{"key":"e_1_2_5_8_2","doi-asserted-by":"crossref","first-page":"14518","DOI":"10.1016\/S0021-9258(18)77333-X","article-title":"ATP synthase complex from bovine heart mitochondria. Subunit arrangement as revealed by nearest neighbor analysis and susceptibility to trypsin","volume":"265","author":"Joshi S","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02555"},{"key":"e_1_2_5_10_2","first-page":"20","article-title":"Understanding network topology","volume":"18","author":"Perkel JM","year":"2004","journal-title":"Scientist"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410397200"}],"container-title":["Proteins: Structure, Function, and Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprot.20600","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prot.20600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T18:07:37Z","timestamp":1697220457000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prot.20600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,3]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1002\/prot.20600"],"URL":"https:\/\/doi.org\/10.1002\/prot.20600","archive":["Portico"],"relation":{},"ISSN":["0887-3585","1097-0134"],"issn-type":[{"value":"0887-3585","type":"print"},{"value":"1097-0134","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,3]]}}}