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By structural alignment with two Sigma class GSTs, glutathione could be rationally docked into the G\u2010site of the enzyme. By comparing with all mammalian GST classes, a novel, long, and deep cleft was identified at the H\u2010site, providing a potential site for ligand binding. This new GST class may support the establishment of infection parasitic nematodes by passively neutralizing chemical toxins derived from host environment. The structure serves as a starting point for structure\u2010based drug\/inhibitor design that would aim to selectively disrupt nematode chemical defenses. 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