{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:42:06Z","timestamp":1791114126388,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The nuclear proteins TAL1 (T\u2010cell acute leukaemia protein 1) and LMO2 (LIM\u2010only protein 2) have critical roles in haematopoietic development, but are also often aberrantly activated in T\u2010cell acute lymphoblastic leukaemia. TAL1 and LMO2 operate within multifactorial protein\u2013DNA complexes that regulate gene expression in the developing blood cell. TAL1 is a tissue\u2010specific basic helix\u2013loop\u2013helix (bHLH) protein that binds bHLH domains of ubiquitous E\u2010proteins, (E12 and E47), to bind E\u2010box (CANNTG) DNA motifs. TAL1<jats:sub>bHLH<\/jats:sub> also interacts specifically with the LIM domains of LMO2, which in turn bind Ldb1 (LIM\u2010domain binding protein 1). Here we used biophysical methods to characterize the assembly of a five\u2010component complex containing TAL1, LMO2, Ldb1, E12, and DNA. The bHLH domains of TAL1 and E12 alone primarily formed helical homodimers, but together preferentially formed heterodimers, to which LMO2 bound with high affinity (<jats:italic>K<\/jats:italic><jats:sub>A<\/jats:sub> \u223c 10<jats:sup>8<\/jats:sup><jats:italic>M<\/jats:italic><jats:sup>\u22121<\/jats:sup>). The resulting TAL1\/E12\/LMO2 complex formed in the presence or absence of DNA, but the different complexes preferentially bound different Ebox\u2010sequences. Our data provide biophysical evidence for a mechanism, by which LMO2 and TAL1 both regulate transcription in normal blood cell development, and synergistically disrupt E2A function in T\u2010cells to promote the onset of leukaemia. Proteins 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.21638","type":"journal-article","created":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T21:46:44Z","timestamp":1191275204000},"page":"1461-1474","source":"Crossref","is-referenced-by-count":24,"title":["Assembly of the oncogenic DNA\u2010binding complex LMO2\u2010Ldb1\u2010TAL1\u2010E12"],"prefix":"10.1002","volume":"70","author":[{"given":"Daniel P.","family":"Ryan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaimee L.","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip W.","family":"Kuchel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline 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