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Here we show that monoclonal antibody 54\u20105C10\u2010A, which is used to diagnose schistosomiasis in humans, interacts with oligomers of at least three Lewis X trisaccharides, but not with monomeric Lewis X. We describe the sequence and the 2.5 \u00c5 crystal structure of its Fab fragment and infer a possible mode of binding of the polymeric Lewis X from docking studies. Our studies indicate a radically different mode of binding compared to Fab 291\u20102G3\u2010A, which is specific for monomeric Lewis X, thus providing a structural explanation of the diagnostic success of 54\u20105C10\u2010A. Proteins 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.22356","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T19:38:24Z","timestamp":1230061104000},"page":"439-447","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of a diagnostic Fab fragment binding trimeric Lewis X"],"prefix":"10.1002","volume":"76","author":[{"given":"Dani\u00ebl C.","family":"de Geus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Marie M.","family":"van Roon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen A. 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