{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T04:54:40Z","timestamp":1696481680336},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:00:00Z","timestamp":1377216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Basic Science Research Program","award":["2008-1617-1","2012-0003936"],"award-info":[{"award-number":["2008-1617-1","2012-0003936"]}]},{"name":"MEST, NRF NCRC","award":["2012-0000952"],"award-info":[{"award-number":["2012-0000952"]}]},{"name":"KOSEF\/MEST","award":["2011-0012456"],"award-info":[{"award-number":["2011-0012456"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The nitrogen\u2010related phosphoenolpyruvate phosphotransferase system (PTS<jats:sup>Ntr<\/jats:sup>) is involved in controlling ammonia assimilation and nitrogen fixation. The additional role of PTS<jats:sup>Ntr<\/jats:sup> as a regulatory link between nitrogen and carbon utilization in <jats:italic>Escherichia coli<\/jats:italic> is assumed to be closely related to molecular functions of IIA<jats:sup>Ntr<\/jats:sup> in potassium homeostasis. We have determined the crystal structure of IIA<jats:sup>Ntr<\/jats:sup> from <jats:italic>Burkholderia pseudomallei<\/jats:italic> (<jats:italic>Bp<\/jats:italic>IIA<jats:sup>Ntr<\/jats:sup>), which is a causative agent of melioidosis. The crystal structure of dimeric <jats:italic>Bp<\/jats:italic>IIA<jats:sup>Ntr<\/jats:sup> determined at 3.0 \u00c5 revealed that its active sites are mutually blocked. This dimeric state is stabilized by charge and weak hydrophobic interactions. Overall monomeric structure and the active site residues, Arg51 and His67, of <jats:italic>Bp<\/jats:italic>IIA<jats:sup>Ntr<\/jats:sup> are well conserved with those of IIA<jats:sup>Ntr<\/jats:sup> enzymes from <jats:italic>E. coli<\/jats:italic> and <jats:italic>Neisseria meningitides<\/jats:italic>. Interestingly, His113 of <jats:italic>Bp<\/jats:italic>IIA<jats:sup>Ntr<\/jats:sup>, which corresponds to a key residue in another phosphoryl group relay in the mannitol\u2010specific enzyme EIIA family (EIIA<jats:sup>Mtl<\/jats:sup>), is located away from the active site due to the loop connecting \u03b25 and \u03b13. Combined with other differences in molecular surface properties, these structural signatures distinguish the IIA<jats:sup>Ntr<\/jats:sup> family from the EIIA<jats:sup>Mtl<\/jats:sup> family. Since, there is no gene for NPr in the chromosome of <jats:italic>B. pseudomallei<\/jats:italic>, modeling and docking studies of the <jats:italic>Bp<\/jats:italic>IIA<jats:sup>Ntr<\/jats:sup>\u2013<jats:italic>Bp<\/jats:italic>HPr complex has been performed to support the proposal on the NPr\u2010like activity of <jats:italic>Bp<\/jats:italic>HPr. A potential dual role of <jats:italic>Bp<\/jats:italic>HPr as a nonspecific phosphocarrier protein interacting with both sugar EIIAs and IIA<jats:sup>Ntr<\/jats:sup> in <jats:italic>B. pseudomallei<\/jats:italic> has been discussed. Proteins 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/prot.24275","type":"journal-article","created":{"date-parts":[[2013,3,12]],"date-time":"2013-03-12T22:32:35Z","timestamp":1363127555000},"page":"1499-1508","source":"Crossref","is-referenced-by-count":2,"title":["New molecular interaction of IIA<sup>Ntr<\/sup> and HPr from <i>Burkholderia pseudomallei<\/i> identified by X\u2010ray crystallography and docking studies"],"prefix":"10.1002","volume":"81","author":[{"given":"Mi\u2010Sun","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Life &amp; Pharmaceutical Sciences The Center for Cell Signaling &amp; Drug Discovery Research College of Pharmacy, Ewha Womans University Seoul 120\u2010750 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasup","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University Seoul 151\u2010747 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lim","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University Seoul 151\u2010747 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areum","family":"Lim","sequence":"additional","affiliation":[{"name":"Division of Life &amp; Pharmaceutical Sciences The Center for Cell Signaling &amp; Drug Discovery Research College of Pharmacy, Ewha Womans University Seoul 120\u2010750 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaok","family":"Seok","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Seoul National University Seoul 151\u2010747 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hae","family":"Shin","sequence":"additional","affiliation":[{"name":"Division of Life 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