{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:12:22Z","timestamp":1780377142627,"version":"3.54.1"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,20]],"date-time":"2013-04-20T00:00:00Z","timestamp":1366416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"crossref","award":["Y1-CO-1020"],"award-info":[{"award-number":["Y1-CO-1020"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["Y1-GM-1104"],"award-info":[{"award-number":["Y1-GM-1104"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"US Department of Energy","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}]},{"name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The NLRP1 inflammasome responds to microbial challenges such as <jats:italic>Bacillus anthracis<\/jats:italic> infection and is implicated in autoimmune disease such as vitiligo. Human NLRP1 contains both an N\u2010terminal pyrin domain (PYD) and a C\u2010terminal caspase recruitment domain (CARD), with the latter being essential for its association with the downstream effector procaspase\u20101. Here we report a 2.0 \u00c5 crystal structure of the human NLRP1 CARD as a fusion with the maltose\u2010binding protein. The structure reveals the six\u2010helix bundle fold of the NLRP1 CARD, typical of the death domain superfamily. The charge surface of the NLRP1 CARD structure and a procaspase\u20101 CARD model suggests potential mechanisms for their association through electrostatic attraction. Proteins 2013; 81:1266\u20131270. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/prot.24287","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T17:11:15Z","timestamp":1363626675000},"page":"1266-1270","source":"Crossref","is-referenced-by-count":64,"title":["Structure of the NLRP1 caspase recruitment domain suggests potential mechanisms for its association with procaspase\u20101"],"prefix":"10.1002","volume":"81","author":[{"given":"Tengchuan","family":"Jin","sequence":"first","affiliation":[{"name":"Structural Immunobiology Unit, Laboratory of Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda Maryland 20892\u20100430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Curry","sequence":"additional","affiliation":[{"name":"Structural Immunobiology Unit, Laboratory of Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda Maryland 20892\u20100430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Smith","sequence":"additional","affiliation":[{"name":"Structural Immunobiology Unit, Laboratory of Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda Maryland 20892\u20100430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Structural Immunobiology Unit, Laboratory of Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda Maryland 20892\u20100430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Sam","family":"Xiao","sequence":"additional","affiliation":[{"name":"Structural Immunobiology Unit, Laboratory of Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda Maryland 20892\u20100430"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,20]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101405"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa061592"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1724"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00599-3"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.01.032"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01043-8"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/21124"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-8-11"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444903008126"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7761829"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.5"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141656"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq366"}],"container-title":["Proteins: Structure, Function, and Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprot.24287","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprot.24287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prot.24287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T02:41:42Z","timestamp":1696473702000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prot.24287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,20]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/prot.24287"],"URL":"https:\/\/doi.org\/10.1002\/prot.24287","archive":["Portico"],"relation":{},"ISSN":["0887-3585","1097-0134"],"issn-type":[{"value":"0887-3585","type":"print"},{"value":"1097-0134","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,20]]}}}