{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:55:20Z","timestamp":1789847720199,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,20]],"date-time":"2013-04-20T00:00:00Z","timestamp":1366416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Protein Structure Initiative project, Enzyme Discovery for Natural Product Biosynthesis (NATPRO) with NIH\/NIGMS","award":["U01GM098248"],"award-info":[{"award-number":["U01GM098248"]}]},{"name":"NIH MERIT Award"},{"name":"U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The molecule known as SF2575 from <jats:italic>Streptomyces sp<\/jats:italic>. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 <jats:italic>in vivo<\/jats:italic>. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial <jats:italic>C<\/jats:italic>\u2010glycosyltransferase (<jats:italic>C<\/jats:italic>\u2010GT) that forms the C\u2010C glycosidic bond between the sugar and the SF2575 tetracycline\u2010like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 \u00c5 resolution. The structures reveal SsfS6 to adopt a GT\u2010B fold wherein the TDP and docked putative aglycon are consistent with the overall <jats:italic>C<\/jats:italic>\u2010glycosylation reaction. As one of only a few existing structures for <jats:italic>C<\/jats:italic>\u2010glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer <jats:italic>C<\/jats:italic>\u2010glycosylation. Proteins 2013; 81:1277\u20131282. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/prot.24289","type":"journal-article","created":{"date-parts":[[2013,3,23]],"date-time":"2013-03-23T09:00:27Z","timestamp":1364029227000},"page":"1277-1282","source":"Crossref","is-referenced-by-count":27,"title":["Crystal structure of SsfS6, the putative <i>C<\/i>\u2010glycosyltransferase involved in SF2575 biosynthesis"],"prefix":"10.1002","volume":"81","author":[{"given":"Fengbin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Cell Biology Rice University Houston Texas 77005"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maoquan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanteri","family":"Singh","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical 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