{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T06:12:29Z","timestamp":1696486349827},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T00:00:00Z","timestamp":1459987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270780","U1332137","31500601"],"award-info":[{"award-number":["31270780","U1332137","31500601"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>TbTFIIS2\u20101, one of the two TFIIS homologues of <jats:italic>Trypanosome brucei<\/jats:italic> (<jats:italic>T. brucei<\/jats:italic>), cooperates with TbTFIIS1 in regulating transcription in <jats:italic>T. brcuei<\/jats:italic>. Structurally divergent from other TFIIS homologues from higher organisms, TbTFIIS2\u20101 contains an additional N\u2010terminal PWWP domain besides other three conserved domains, which may imply potential role of TbTFIIS2\u20101 in transcription regulation. Here, we determined the solution structure of PWWP domain of TbTFIIS2\u20101 by NMR spectroscopy, which was the first solution structure of PWWP domain solved in trypanosomatid. In spite of poor sequence similarity between PWWP domains, this domain of TbTFIIS2\u20101 adopts a conserved 3D\u2010structure, which contains a five\u2010stranded \u03b2\u2010barrel and a C\u2010terminal \u03b1\u2010helix. Furthermore, we found that TbTFIIS2\u20101 PWWP domain may be a protein\u2013protein interaction module without the ability of DNA recognition and methyl\u2010group interaction. Proteins 2016; 84:912\u2013919. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/prot.25035","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T08:32:58Z","timestamp":1458721978000},"page":"912-919","source":"Crossref","is-referenced-by-count":2,"title":["Solution structure of TbTFIIS2\u20101 PWWP domain from <i>Trypanosoma brucei<\/i>"],"prefix":"10.1002","volume":"84","author":[{"given":"Rui","family":"Wang","sequence":"first","affiliation":[{"name":"Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China Hefei Anhui 230026 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China Hefei Anhui 230026 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanhui","family":"Liao","sequence":"additional","affiliation":[{"name":"Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China Hefei Anhui 230026 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Tu","sequence":"additional","affiliation":[{"name":"Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China Hefei Anhui 230026 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704534104"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704573104"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.15.8991"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2003.09.005"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1112680"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06348.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.7.1071"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018919"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01449-6"},{"key":"e_1_2_5_11_1","first-page":"217","article-title":"The PWWP domain 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