{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:41:02Z","timestamp":1778834462247,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["RR\u201001646"],"award-info":[{"award-number":["RR\u201001646"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["GSP\u201048370"],"award-info":[{"award-number":["GSP\u201048370"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Legionella pneumophila<\/jats:italic> is a pathogen causing severe pneumonia in humans called Legionnaires\u2019 disease. Lem22 is a previously uncharacterized effector protein conserved in multiple Legionella strains. Here, we report the crystal structure of Lem22 from the Philadelphia strain, also known as lpg2328, at 1.40 \u00c5 resolution. The structure shows an up\u2010and\u2010down three\u2010helical bundle with a significant structural similarity to a number of protein\u2010binding domains involved in apoptosis and membrane trafficking. Sequence conservation identifies a putative functional site on the interface of helices 2 and 3. The structure is an important step toward a functional characterization of Lem22.<\/jats:p>","DOI":"10.1002\/prot.25427","type":"journal-article","created":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T09:36:19Z","timestamp":1511256979000},"page":"263-267","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of the Legionella effector Lem22"],"prefix":"10.1002","volume":"86","author":[{"given":"Guennadi","family":"Kozlov","sequence":"first","affiliation":[{"name":"Department of Biochemistry Groupe de recherche ax\u00e9 sur la structure des prot\u00e9ines, McGill University Montreal QC H3G 0B1 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6109-0650","authenticated-orcid":false,"given":"Kathy","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Groupe de recherche ax\u00e9 sur la structure des prot\u00e9ines, McGill University Montreal QC H3G 0B1 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalle","family":"Gehring","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Groupe de recherche ax\u00e9 sur la structure des prot\u00e9ines, McGill University Montreal QC H3G 0B1 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5352.873"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.053579"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000508"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00208"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3443"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001269107"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5512"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2011.07.005"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911001314"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(03)74014-2"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906005270"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq366"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.371492"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01490-X"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913003624"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.276"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.2.781"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2610"}],"container-title":["Proteins: Structure, Function, and Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprot.25427","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prot.25427","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T15:51:14Z","timestamp":1695916274000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prot.25427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,29]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/prot.25427"],"URL":"https:\/\/doi.org\/10.1002\/prot.25427","archive":["Portico"],"relation":{},"ISSN":["0887-3585","1097-0134"],"issn-type":[{"value":"0887-3585","type":"print"},{"value":"1097-0134","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,29]]}}}