{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:41:16Z","timestamp":1778658076615,"version":"3.51.4"},"reference-count":79,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We investigated the structure and Brownian rotational motion of the PEST region (201\u2010268) from human c\u2010Myc oncoprotein, whose overexpression\/dysregulation is associated with various types of cancer. The 77\u2010residue PEST fragment revealed a large Stokes radius (~3.1 nm) and CD spectrum highlighting abundance of disordered structure. Changes in structure\/dynamics at two specific sites in PEST degron were observed using time\u2010resolved fluorescence spectroscopy by labeling Cys\n                    <jats:sup>9<\/jats:sup>\n                    near N\u2010terminal with dansyl probe and inserting a Trp\n                    <jats:sup>70<\/jats:sup>\n                    near C\u2010terminal (PEST M1). Trp in PEST M1 at pH 3 was inaccessible to quencher, showed hindered segmental motion and slow global rotation (~30\u2009ns) in contrast to N\u2010terminal where the dansyl probe was free, exposed with fast global rotation (~5 ns). Remarkably, this large monomeric structure at acidic pH was retained irrespective of ionic strength (0.03\u20100.25\u2009M) and partially so in presence of 6 M Gdn.HCl. With gradual increase in pH, a structural transition (~pH 4.8) into a more exposed and freely rotating Trp was noticeable. Interestingly, the induced structure at C\u2010terminal also influenced the dynamics of dansyl probe near N\u2010terminal, which otherwise remained unstructured at pH\u2009&gt;\u20095. FRET measurements confirmed a 11\u2009\u00c5 decrease in distance between dansyl and indole at pH 4 compared to pH 9, coinciding with enhanced ANS binding and increase in strand\/helix population in both PEST fragments. The protonation of glutamate\/aspartate residues in C\u2010terminal region of PEST is implicated in this disorder\u2010order transition. This may have a bearing on the role of PEST in endocytic trafficking of eukaryotic proteins.\n                  <\/jats:p>","DOI":"10.1002\/prot.25880","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T10:06:36Z","timestamp":1580378796000},"page":"889-909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Structure and dynamics at N\u2010 and C\u2010terminal regions of intrinsically disordered human c\u2010Myc PEST degron reveal a pH\u2010induced transition"],"prefix":"10.1002","volume":"88","author":[{"given":"Mohd. Ziauddin","family":"Ansari","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati  Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1294-8379","authenticated-orcid":false,"given":"Rajaram","family":"Swaminathan","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati  Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60353-X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.61.070192.004113"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.653"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.08.033"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.1.1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4417"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2006410"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60476-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200828"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.12.7106"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(94)90502-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2221"},{"key":"e_1_2_9_14_1","first-page":"2649","article-title":"In vivo ubiquitination and proteasome\u2010mediated degradation of p53","volume":"56","author":"Maki CG","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.7.2423-2435.2000"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.3.717"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.6.2104"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2876518"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)10031-1"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb814"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.73"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03481.x"},{"key":"e_1_2_9_23_1","first-page":"3211","article-title":"Identification of casein kinase II phosphorylation sites in Max: effects on DNA\u2010binding kinetics of Max homo\u2010and Myc\/Max heterodimers","volume":"8","author":"Bousset K","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2005.11.012"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.271528"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0410"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.12.7953"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.6.3021"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.10.014"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aak9982"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029199"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112037200"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20746"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100088a030"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-211"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0134(20001115)41:3<415::AID-PROT130>3.0.CO;2-7"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-111"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050082"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7FD00194K"},{"key":"e_1_2_9_40_1","first-page":"1276","article-title":"The molecular probes handbook. 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