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However, the widespread use of these polyphenol oxidases is limited by elevated production costs and restricted knowledge on their spectrum of action. Here, six tyrosinase homologs were identified and characterized from the genomes of four widespread freshwater ciliates using bioinformatics. Next, we performed a virtual screening to calculate binding energies between 3D models of these homologs and ~\u20091000 contaminants of emerging concern (CECs), as an indirect approach to identify likely and unlikely targets for tyrosinases. Many fine chemicals, pharmaceuticals, personal care products, illicit drugs, natural toxins, and pesticides exhibited strong binding energies to these new tyrosinases, suggesting the spectrum of targets of these enzymes might be considerably broader than previously thought. Many ciliates, including those carrying tyrosinase genes, are fast\u2010growing unicellular microeukaryotes that can be efficiently cultured, at large scales, under in vitro conditions, suggesting these organisms should be regarded as potential low\u2010cost sources of new environmental biotechnological molecules.<\/jats:p>","DOI":"10.1002\/prot.26139","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T02:46:13Z","timestamp":1620614773000},"page":"1180-1192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New tyrosinases with putative action against contaminants of emerging concern"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3866-8837","authenticated-orcid":false,"given":"Marcus Vinicius","family":"Xavier Senra","sequence":"first","affiliation":[{"name":"Instituto de Recursos Naturais Universidade Federal de Itajub\u00e1  Itajub\u00e1 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