{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:28:36Z","timestamp":1788449316202,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["project CALIPSOplus under the Grant Agreement 7308"],"award-info":[{"award-number":["project CALIPSOplus under the Grant Agreement 7308"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004271","name":"Sapienza Universit\u00e0 di Roma","doi-asserted-by":"publisher","award":["RP11916B407928AA"],"award-info":[{"award-number":["RP11916B407928AA"]}],"id":[{"id":"10.13039\/501100004271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    is a saprophytic ubiquitous fungus whose spores can trigger reactions such as allergic bronchopulmonary aspergillosis or the fatal invasive pulmonary aspergillosis. To survive in the lungs, the fungus must adapt to a hypoxic and nutritionally restrictive environment, exploiting the limited availability of aromatic amino acids (AAAs) in the best possible way, as mammals do not synthesize them. A key enzyme for AAAs catabolism in\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    is AroH, a pyridoxal 5\u2032\u2010phosphate\u2010dependent aromatic aminotransferase. AroH was recently shown to display a broad substrate specificity, accepting L\u2010kynurenine and \u03b1\u2010aminoadipate as amino donors besides AAAs. Given its pivotal role in the adaptability of the fungus to nutrient conditions, AroH represents a potential target for the development of innovative therapies against\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    \u2010related diseases. We have solved the crystal structure of\n                    <jats:italic>Af\u2010<\/jats:italic>\n                    AroH at 2.4\u00a0\u00c5 resolution and gained new insight into the dynamics of the enzyme's active site, which appears to be crucial for the design of inhibitors. The conformational plasticity of the active site pocket is probably linked to the wide substrate specificity of AroH.\n                  <\/jats:p>","DOI":"10.1002\/prot.26234","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T11:00:00Z","timestamp":1631098800000},"page":"435-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Crystal structure of\n                    <i>Aspergillus fumigatus<\/i>\n                    <scp>AroH<\/scp>\n                    , an aromatic amino acid aminotransferase"],"prefix":"10.1002","volume":"90","author":[{"given":"Sharon","family":"Spizzichino","sequence":"first","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gioena","family":"Pampalone","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Perugia Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirco","family":"Dindo","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Perugia Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agostino","family":"Bruno","sequence":"additional","affiliation":[{"name":"Food and Drug Department University of Parma Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigina","family":"Romani","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Perugia Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4621-2135","authenticated-orcid":false,"given":"Francesca","family":"Cutruzzol\u00e0","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Zelante","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Perugia Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Pieroni","sequence":"additional","affiliation":[{"name":"Food and Drug Department University of Parma Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Cellini","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Perugia Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0802-1370","authenticated-orcid":false,"given":"Giorgio","family":"Giardina","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Sapienza University of Rome Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802139867"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2017.01996","article-title":"A multifaceted role of tryptophan metabolism and indoleamine 2,3\u2010dioxygenase activity in Aspergillus fumigatus\u2010host interactions","volume":"8","author":"Choera T","year":"2018","journal-title":"Front 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