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There are no effective antiviral drugs used to control and treat PCV2, and prevention is mainly obtained through vaccination. PCV2 genome replicates through the rolling circle replication (RCR) mechanism involving Rep and Rep\u2032, so analyzing the holistic structure of Rep and Rep\u2032 will help us better understand the replication process of PCV2. However, there are no reports on the integral structure of Rep\u2032 and Rep, which seriously hinders the research of the viral replication. By using the x\u2010ray diffraction method, the structure of the Rep\u2032 dimer was resolved by us in this study. Structural analysis revealed that Rep\u2032 is a dimer formed by the interaction of the C\u2010terminal domain. The two Rep\u2032 form a positively charged groove, which may play an essential role in the viral binding of dsDNA. Together, this study help to understand the replication process of the virus and may also provide new insights into the development of antiviral drugs.<\/jats:p>","DOI":"10.1002\/prot.26498","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T08:14:30Z","timestamp":1683879270000},"page":"1130-1139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Crystal structure of the dimerized of porcine circovirus type\n                    <scp>II<\/scp>\n                    replication\u2010related protein Rep\u2032"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-2332","authenticated-orcid":false,"given":"Shuaiyin","family":"Guan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University  Wuhan China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University  Wuhan 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