{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T21:56:37Z","timestamp":1780350997193,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008783","name":"National Research Council of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008783","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The activation or inactivation of B\u2010cell lymphoma\u20102 (Bcl\u20102) antagonist\/killer (Bak) is critical for controlling mitochondrial outer membrane permeabilization\u2010dependent apoptosis. Its pro\u2010apoptotic activity is controlled by intermolecular interactions with the Bcl\u20102 homology 3 (BH3) domain, which is accommodated in the hydrophobic pocket of Bak. Bcl\u20102\u2010interacting protein 5 (Bnip5) is a noncanonical BH3 domain\u2010containing protein that interacts with Bak. Bnip5 is characterized by its controversial effects on the regulation of the pro\u2010apoptotic activity of Bak. In the present study, we determined the crystal structure of Bak bound to Bnip5 BH3. The intermolecular association appeared to be typical at first glance, but we found that it is maintained by tight hydrophobic interactions together with hydrogen\/ionic bonds, which accounts for their high binding affinity with a dissociation constant of 775\u2009nM. Structural analysis of the complex showed that Bnip5 interacts with Bak in a manner similar to that of the Bak\u2010activating pro\u2010apoptotic factor peroxisomal testis\u2010enriched protein 1, particularly in the destabilization of the intramolecular electrostatic network of Bak. Our structure is considered to reflect the initial point of drastic and consecutive conformational and stoichiometric changes in Bak induced by Bnip5 BH3, which helps in explaining the effects of Bnip5 in regulating Bak\u2010mediated apoptosis.<\/jats:p>","DOI":"10.1002\/prot.26568","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T02:31:34Z","timestamp":1691548294000},"page":"44-51","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Crystal structure of Bak bound to the\n                    <scp>BH3<\/scp>\n                    domain of Bnip5, a noncanonical\n                    <scp>BH3<\/scp>\n                    domain\u2010containing protein"],"prefix":"10.1002","volume":"92","author":[{"given":"Dahwan","family":"Lim","sequence":"first","affiliation":[{"name":"Disease Target Structure Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"},{"name":"Department of Biochemistry Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Eun","family":"Jeong","sequence":"additional","affiliation":[{"name":"Disease Target Structure Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"},{"name":"Department of Biology Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho\u2010Chul","family":"Shin","sequence":"additional","affiliation":[{"name":"Critical Diseases Diagnostics Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joon Sig","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinho","family":"Seo","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Jun","family":"Kim","sequence":"additional","affiliation":[{"name":"Disease Target Structure Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"},{"name":"Critical Diseases Diagnostics Convergence Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1784-8975","authenticated-orcid":false,"given":"Bonsu","family":"Ku","sequence":"additional","affiliation":[{"name":"Disease Target Structure Research Center Korea Research Institute of Bioscience and Biotechnology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14186"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059108"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a036319"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.149"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.12.030"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2563"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27851-y"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.11.001"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2023.01.001"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002156"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003693"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.08.009"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203724666230314164040"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1101\/2022.07.24.499430"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.07.016"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"}],"container-title":["Proteins: Structure, Function, and 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