{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:46:57Z","timestamp":1788410817854,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:00:00Z","timestamp":1705449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004505","name":"Universit\u00e0 di Catania","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201010\u2010INBS\u201004\u201001"],"award-info":[{"award-number":["ANR\u201010\u2010INBS\u201004\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Phospholipids in biological membranes establish a chemical equilibrium between free phospholipids in the aqueous phase (CMC) and self\u2010assembled phospholipids in vesicles, keeping the CMC constant. The CMC is different for each phospholipid, depends on the amount of cholesterol, and, according to the lipid\u2010chaperone hypothesis, controls the interaction between free phospholipids and amyloidogenic proteins (such as amylin, amyloid\u2010\u03b2, and \u03b1\u2010synuclein, all of which are, respectively, associated with a different proteinopathy), which governs the formation of a toxic complex between free lipids and proteins that leads to membrane destruction. Here, we provide quantitative measurements of CMCs and bilayer stability of pure phospholipids, lipid rafts, and their mixture with cholesterol by fluorescence methods (using pyrene as a probe) and light scattering techniques (resonance Rayleigh scattering and fixed\u2010angle light scattering) performed on LUVs, as well as AFM to measure LUV dimensions. Also, we test the lipid\u2010chaperone hypothesis on human IAPP interacting with different mixture of POPC cholesterol. Stated the importance of CMC in membrane stability and protein aggregation processes, these results could be a starting point for the development of a quantitative kinetic model for the lipid chaperone hypothesis.<\/jats:p>","DOI":"10.1002\/prot.26669","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T01:58:36Z","timestamp":1705543116000},"page":"1383-1392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Critical micellar concentration determination of pure phospholipids and lipid\u2010raft and their mixtures with cholesterol"],"prefix":"10.1002","volume":"93","author":[{"given":"Sofia","family":"Serravalle","sequence":"first","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Pisano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele F. M.","family":"Sciacca","sequence":"additional","affiliation":[{"name":"Istituto di Cristallografia, Consiglio Nazionale delle Ricerche  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Salamone","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Sicali","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Mazzara","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Costa","sequence":"additional","affiliation":[{"name":"Centre de Biologie Structurale University of Montpellier, CNRS, INSERM  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7123-5347","authenticated-orcid":false,"given":"Carmelo","family":"La Rosa","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Catania  Catania 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