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In addition, the infection may further lead to the failure of an immune system completely. The present study aimed to propose a possible vaccine by employing bioinformatics techniques to identify B and T\u2010cell antigens. The best multi\u2010epitope of potential immunogenicity was generated by combining epitopes. Additionally, the linkers EAAAK, AAY, and GPGPG were utilized in order to link the epitopes successfully. Further, C\u2010ImmSim was used to perform in silico immunological simulations upon the vaccine. For the purpose of conducting expression tests in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , the chimeric protein construct was cloned using Snapgene into the pET\u20109c vector. The designed vaccine showed adequate results, evidenced by the global population coverage and favorable immune response. The developed vaccine was found to be highly effective and to have excellent population coverage in a number of computer\u2010based assessments. This work is fully dependent on the development of nucleoprotein\u2010based vaccines, which would constitute a significant step forward if our findings were used in developing a global vaccination to combat the Puumala virus.\n                  <\/jats:p>","DOI":"10.1002\/prot.26703","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T09:26:10Z","timestamp":1715678770000},"page":"1161-1176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<i>In silico<\/i>\n                    design and evaluation of a multiepitope vaccine targeting the nucleoprotein of\n                    <i>Puumala orthohantavirus<\/i>"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5546-1504","authenticated-orcid":false,"given":"Kunal","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"Pratiksha Institute of Pharmaceutical Sciences  Guwahati Assam 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