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They all contain similar\n                    <jats:italic>lux<\/jats:italic>\n                    operons, which include the genes\n                    <jats:italic>luxA<\/jats:italic>\n                    and\n                    <jats:italic>luxB<\/jats:italic>\n                    encoding a heterodimeric luciferase. The aldehyde oxygenation reaction is presumed to be catalyzed primarily by the subunit LuxA, whereas LuxB is required for efficiency and stability of the complex. Recently, genomic analysis identified a subset of bacterial species with rearranged\n                    <jats:italic>lux<\/jats:italic>\n                    operons lacking\n                    <jats:italic>luxB<\/jats:italic>\n                    . Here, we show that the product of the\n                    <jats:italic>luxA<\/jats:italic>\n                    gene from the reduced\n                    <jats:italic>luxACDE<\/jats:italic>\n                    operon of\n                    <jats:italic>Enhygromyxa salina<\/jats:italic>\n                    is luminescent upon addition of aldehydes both in vivo in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and in vitro. Overall,\n                    <jats:italic>Es<\/jats:italic>\n                    LuxA is much less bright compared with luciferases from\n                    <jats:italic>Aliivibrio fischeri<\/jats:italic>\n                    (\n                    <jats:italic>Af<\/jats:italic>\n                    LuxAB) and\n                    <jats:italic>Photorhabdus luminescens<\/jats:italic>\n                    (\n                    <jats:italic>Pl<\/jats:italic>\n                    LuxAB), and most active with medium\u2010chain C4\u2013C9 aldehydes. Crystal structure of\n                    <jats:italic>Es<\/jats:italic>\n                    LuxA determined at the resolution of 2.71\u2009\u00c5 reveals a (\u03b2\/\u03b1)\n                    <jats:sub>8<\/jats:sub>\n                    TIM\u2010barrel fold, characteristic for other bacterial luciferases, and the protein preferentially forms a dimer in solution. The mobile loop residues 264\u2013293, which form a \u03b2\u2010hairpin or a coil in\n                    <jats:italic>Vibrio harveyi<\/jats:italic>\n                    LuxA, form \u03b1\u2010helices in\n                    <jats:italic>Es<\/jats:italic>\n                    LuxA. Phylogenetic analysis shows\n                    <jats:italic>Es<\/jats:italic>\n                    LuxA and related proteins may be bacterial protoluciferases that arose prior to duplication of the\n                    <jats:italic>luxA<\/jats:italic>\n                    gene and its speciation to\n                    <jats:italic>luxA<\/jats:italic>\n                    and\n                    <jats:italic>luxB<\/jats:italic>\n                    in the previously described luminescent bacteria. Our work paves the way for the development of new bacterial luciferases that have an advantage of being encoded by a single gene.\n                  <\/jats:p>","DOI":"10.1002\/prot.26739","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T13:36:53Z","timestamp":1724333813000},"page":"1449-1458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>luxA<\/i>\n                    Gene From\n                    <i>Enhygromyxa salina<\/i>\n                    Encodes a Functional Homodimeric Luciferase"],"prefix":"10.1002","volume":"92","author":[{"given":"Anna","family":"Yudenko","sequence":"first","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey V.","family":"Bazhenov","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir A.","family":"Aleksenko","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan M.","family":"Goncharov","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg","family":"Semenov","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Remeeva","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera V.","family":"Nazarenko","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizaveta","family":"Kuznetsova","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim V.","family":"Fomin","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria N.","family":"Konopleva","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahaf","family":"Al Ebrahim","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Mechanisms of Aging and Age\u2010Related Diseases Moscow Institute of Physics and Technology  Dolgoprudny Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai N.","family":"Sluchanko","sequence":"additional","affiliation":[{"name":"A.N. 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