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Simultaneously, while half of the world's population depends on rice (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oryza sativa<\/jats:italic>\n                    <\/jats:styled-content>\n                    L.) as a staple food, this cereal does not provide a sufficient amount of that micronutrient to meet these people's nutritional needs: even when iron is readily available in the soil, it does not accumulate in the consumed portion of the grain, namely, the starchy endosperm, being instead retained in the aleurone layer, in the pericarp and in the embryo. In this context, the present work applies computational biology tools\u2014such as normal mode analysis and molecular dynamics simulations\u2014to elucidate the behavior and transport mechanism of the Vacuolar Iron Transporter 2 (OsVIT2), a central protein for iron homeostasis in rice, with the objective of laying the foundations for future OsVIT2 engineering projects that could be articulated with ongoing efforts to promote iron biofortification in rice. We shed light on the interplay between protonation state, configuration and hydration of OsVIT2's pore; on the mechanics of its opening and on the ever\u2010shifting hydrogen bond network contained within it. We also explore the potential contribution of the \u201cflexible arms\u201d to the iron\u2010capturing function performed by the cytoplasmic domain.\n                  <\/jats:p>","DOI":"10.1002\/prot.26843","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T01:23:47Z","timestamp":1747358627000},"page":"1717-1731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Basis for Vacuolar Iron Transport by OsVIT2, a Target for Iron Biofortification in Rice"],"prefix":"10.1002","volume":"93","author":[{"given":"L. B.","family":"Arend","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. S.","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. G. S.","family":"Costa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. K.","family":"Ricachenevsky","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-8620","authenticated-orcid":false,"given":"H.","family":"Verli","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c07727"},{"key":"e_1_2_8_3_1","volume-title":"Iron Deficiency Anemia Assessment Prevention and Control: A Guide for Program Managers","author":"World Health Organization","year":"2001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2020.02.021"},{"issue":"2","key":"e_1_2_8_5_1","first-page":"164","article-title":"Review on Iron and Its Importance for Human Health","volume":"19","author":"Abbaspour N.","year":"2014","journal-title":"Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodpol.2013.06.009"},{"key":"e_1_2_8_7_1","first-page":"1","volume-title":"Advances in Agronomy","author":"Graham R. 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