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Using the epidermal growth factor binding protein (mGK\u20109) and the \u03b3\u2010subunit of nerve growth factor (nGK)\u20103, as test cases, mature processed forms, obtained as functionally active proteins, as well as various precursor forms, were isolated. The expression system described allows rapid isolation of kallikrein protein from corresponding cDNA with yields of approximately 1.0 mg of purified protein from 10 g of initial cell paste. This expression system will facilitate structure\/function studies of the mouse glandular kallikrein gene family and help elucidate the regions of the mature proteins responsible for the diverse catalytic behavior and growth factors interactions observed in this family of proteins.<\/jats:p>","DOI":"10.1002\/prot.340070309","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T01:07:09Z","timestamp":1117328829000},"page":"280-290","source":"Crossref","is-referenced-by-count":7,"title":["The characterization of recombinant mouse glandular kallikreins from <i>E. coli<\/i>"],"prefix":"10.1002","volume":"7","author":[{"given":"Michael","family":"Blaber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Isackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph. 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