{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:48:15Z","timestamp":1698144495947},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,3]],"date-time":"2004-02-03T00:00:00Z","timestamp":1075766400000},"content-version":"vor","delay-in-days":4203,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pancreatic spasmolytic polypeptide (PSP) isolated from porcine pancreas has been crystallized by the hanging drop vapor diffusion method. Crystals suitable for X\u2010ray diffraction analysis were grown at pH 4.7 from a solution of 6% saturated ammonium sulfate. The space group is orthorhombic <jats:italic>I<\/jats:italic>222 or <jats:italic>I<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub> with unit cell parameters <jats:italic>a<\/jats:italic> = 54.38 \u00c5, <jats:italic>b<\/jats:italic> = 72.29 \u00c5, and <jats:italic>c<\/jats:italic> = 180.85 \u00c5. There are three molecules of PSP per asymmetric unit and a water content of 46.9%. The crystals diffracts to an estimated resolution of 2.7 \u00c5.<\/jats:p><jats:p>The far\u2010UV CD spectrum of PSP shows some exceptional features which cannot be accounted for thoroughly in terms of standard secondary structures commonly seen in protein CD spectroscopy. With this limitation, the secondary structure analysis predicts 15% \u03b1\u2010helix, between 10 and 20% antiparallel \u03b2\u2010strand, 10% parallel \u03b2\u2010strand, 15% turn, and 25 to 40% of other structures. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.340130408","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T01:19:35Z","timestamp":1117329575000},"page":"364-368","source":"Crossref","is-referenced-by-count":5,"title":["Pancreatic spasmolytic polypeptide: Crystallization, circular dichroism analysis, and preliminary X\u2010ray diffraction studies"],"prefix":"10.1002","volume":"13","author":[{"given":"Michael","family":"Gajhede","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Thim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klavs H.","family":"J\u00f8rgensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steen G.","family":"Melberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(82)90126-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(82)90127-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(85)90226-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(89)90288-4"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80690-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/j.1460-2075.1990.tb08125.x","article-title":"hSP, the domain duplicated homolog of pS2 protein is co\u2010expressed with pS2 in stomach but not in breast carcinoma","volume":"9","author":"Tomassetto C.","year":"1990","journal-title":"EMBO J."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(82)90128-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2530309a"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(86)90028-5"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(88)90056-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","unstructured":"Rasmussen T. N. Thim L. Raabjerg L. Harling H. Holst J. J.Pancreatic spasmolytic polypeptide (PSP) a potential growth factor for the intestine: Localization and control of secretion. In: Creutzfeldt W. Stockmann F. (eds.) \u201c8th International Symposium on Gastrointestinal Hormones.\u201d Timmerdorfer Strand: FRG. Digestion 46:90 1990.","DOI":"10.1159\/000200390"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"7686","DOI":"10.1016\/S0021-9258(18)68553-9","article-title":"A new repetitive protein from xenoplus lae\u2010vis skin highly homologous to pancreatic spasmolytic polypeptide","volume":"263","author":"Hoffmann W.","year":"1988","journal-title":"J. Biol. 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