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These organisms possess distinct Rieske\/cytochrome\n                    <jats:italic>b<\/jats:italic>\n                    complexes whose precise role in anammox metabolism remains unclear, but which have been proposed to include the generation of NAD(P)H. This would require energetics and structural features unusual for such complexes. Here we present crystal structures and electrochemical investigations of the Rieske subunits of two of these complexes from the anammox organism\n                    <jats:italic>Kuenenia stuttgartiensis<\/jats:italic>\n                    , Kuste4569 and Kustd1480. Both proteins display high redox potentials (&gt;\u2009+\u2009300\u2009mV), which can be in part explained by their crystal structures and which fit perfectly into the energetic scheme of the proposed NAD(P)H generation mechanism. Moreover, AlphaFold3 models of the parent complexes trace out a path for the electrons required for NAD(P)H production, which includes a proposed, novel\n                    <jats:italic>b<\/jats:italic>\n                    \u2010type heme in the membrane\u2010bound part of the complex.\n                  <\/jats:p>","DOI":"10.1002\/prot.70084","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T12:11:08Z","timestamp":1762949468000},"page":"905-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rieske Iron\u2013Sulfur Cluster Proteins From an Anaerobic Ammonium Oxidizer Suggest Unusual Energetics in Their Parent Rieske\/Cytochrome\n                    <i>b<\/i>\n                    Complexes"],"prefix":"10.1002","volume":"94","author":[{"given":"David","family":"Hauser","sequence":"first","affiliation":[{"name":"Department of Biomolecular Mechanisms Max Planck Institute for Medical Research  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Sode","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Mechanisms Max Planck Institute for Medical Research  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena A.","family":"Andreeva","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Mechanisms Max Planck Institute for Medical Research  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Parey","sequence":"additional","affiliation":[{"name":"Structural Biology Section, Department of Biology\/Chemistry Osnabr\u00fcck University  Osnabr\u00fcck Germany"},{"name":"Center of Cellular Nanoanalytics Osnabr\u00fcck (CellNanOs) Osnabr\u00fcck University  Osnabr\u00fcck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9488-3005","authenticated-orcid":false,"given":"Thomas R. 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