{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:43:26Z","timestamp":1788317006367,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93022C00036"],"award-info":[{"award-number":["75N93022C00036"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI169344"],"award-info":[{"award-number":["AI169344"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Iron homeostasis in various pathogenic bacteria is regulated by bacterioferritins (Bfr) which function to store Fe\n                    <jats:sup>3+<\/jats:sup>\n                    and release Fe\n                    <jats:sup>2+<\/jats:sup>\n                    as needed for metabolic processes. The Bfr structure consists of 18\u2009kDa subunits in which dimer pairs bind a heme molecule and are assembled into a highly symmetrical 24\u2010meric spherical structure with an internal core diameter of approximately 80\u2009\u00c5. Release of iron is facilitated by the binding of a 7\u2009kDa [2Fe\u20102S] ferredoxin (Bfd) to specific sites on the surface of Bfr which transfers electrons to the core thereby reducing the stored Fe\n                    <jats:sup>3+<\/jats:sup>\n                    to Fe\n                    <jats:sup>2+<\/jats:sup>\n                    for mobilization. The crystal structures of Bfr from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Brucella abortus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:italic>Ba<\/jats:italic>\n                    ) in the apo and iron bound forms are presented and compared with those from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:italic>Ab<\/jats:italic>\n                    ) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:italic>Pa<\/jats:italic>\n                    ). Additionally, models of the Bfr:Bfd complexes for\n                    <jats:italic>Ba<\/jats:italic>\n                    and\n                    <jats:italic>Ab<\/jats:italic>\n                    are provided and compared with the\n                    <jats:italic>Pa<\/jats:italic>\n                    complex. Finally, compounds known to target the Bfr:Bfd interaction in\n                    <jats:italic>Pa<\/jats:italic>\n                    were docked to the\n                    <jats:italic>Ba<\/jats:italic>\n                    and\n                    <jats:italic>Ab<\/jats:italic>\n                    structures which provided insight regarding the potential binding mode and inhibitory mechanism.\n                  <\/jats:p>","DOI":"10.1002\/prot.70109","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T03:43:57Z","timestamp":1767411837000},"page":"1170-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Structural Analysis and Inhibitor Modeling of Bacterioferritin From\n                    <scp>\n                      <i>Brucella abortus<\/i>\n                    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Emerging and re\u2010Emerging Infectious Diseases (CERID), division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Chemistry Louisiana State University  Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Battaile","sequence":"additional","affiliation":[{"name":"New York Structural Biology Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Molecular Graphics and Modeling Laboratory and the Computational Chemical Biology Core University of Kansas  Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Seattle Structural 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