{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:55:53Z","timestamp":1790571353171,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102024\u201005544"],"award-info":[{"award-number":["RGPIN\u20102024\u201005544"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000046","name":"National Research Council Canada","doi-asserted-by":"publisher","award":["AQC\u2010200\u20101"],"award-info":[{"award-number":["AQC\u2010200\u20101"]}],"id":[{"id":"10.13039\/501100000046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Metamorphic proteins challenge the classical view of protein folding by reversibly interconverting between two distinct, stable native structures. XCL1 (lymphotactin) is a prototypical example, transitioning between a monomeric mixed\u2010 chemokine fold and an alternate all\u2010 fold that forms a stable dimer. Here we develop and apply a coarse\u2010grained, structure\u2010based model to investigate how temperature and electrostatic screening control this fold switch. By tuning the relative interaction strength of native contacts in the two folds, we show that the model qualitatively reproduces the experimentally observed temperature dependence of XCL1 fold populations, including an increasing alternate\u2010fold population with increasing temperature. We introduce a sequence\u2010dependent treatment of salt effects, which reveals a strong sensitivity of the fold equilibrium to salt concentration and yields a temperature\u2010salt phase diagram with a broad coexistence region. Focusing on conditions near coexistence, kinetic simulations of the chemokine\u2010to\u2010alternate fold transition show that productive dimerization tends to occur when both chains are at least partially unfolded, and that formation of the final alternate dimer may proceed via an intermediate state characterized by a partially formed dimer interface. Finally, we use the model to examine how charge patterning in ancestral variants of XCL1 modulates the population balance between the two folds.<\/jats:p>","DOI":"10.1002\/prot.70179","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:14:47Z","timestamp":1790568887000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermodynamics and Kinetics of Dimerization\u2010Coupled Fold Switching in the Chemokine\n                    <scp>XCL1<\/scp>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6202-5316","authenticated-orcid":false,"given":"Bahman","family":"Seifi","sequence":"first","affiliation":[{"name":"Department of Physics and Physical Oceanography Memorial University  St. John's Newfoundland 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roteins: 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