{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:39:07Z","timestamp":1792636747686,"version":"4.5.0"},"reference-count":52,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["SRG\/2020\/002186"],"award-info":[{"award-number":["SRG\/2020\/002186"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteins"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    <\/jats:styled-content>\n                    fatty acid synthase I (Mtb FAS\u2010I) is a multifunctional hexameric complex essential for fatty acid (FA) synthesis. The need of a hexameric structure for activity of the complex in Mtb remains elusive. Here, we model a conformation of the functionally active complex with acyl carrier protein (ACP) at ketoacyl synthase (KS). Our model reveals a crucial cross\u2010dome dependence in the mechanism of FA synthesis at the condensation step. Using molecular dynamics simulation, we identify key ACP and KS residues that maintain persistent interactions. ACP's phosphopantetheine (PPT) arm adopts several conformations while accessing KS's catalytic pocket, including two distinct conformations that correlate with volumes of ACP and KS pockets. A PHE residue, reported as a gatekeeper of the KS pocket in other species, also shows open and closed orientations in our simulation. Our results provide crucial insights that are essential for a mechanistic understanding of the Mtb FAS\u2010I complex.\n                  <\/jats:p>","DOI":"10.1002\/prot.70193","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:05:19Z","timestamp":1792634719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modeling Type\u2010I Fatty Acid Synthase With Acyl Carrier Protein at Ketoacyl Synthase"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6444-2690","authenticated-orcid":false,"given":"Manisha","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Indian Institute of Technology Kanpur  Kanpur Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5111-8066","authenticated-orcid":false,"given":"Harshwardhan H.","family":"Katkar","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Indian Institute of Technology Kanpur  Kanpur Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-57418-8","article-title":"Analysis of the Co\u2010Translational Assembly of the Fungal Fatty Acid Synthase (FAS)","volume":"10","author":"Fischer M.","year":"2020","journal-title":"Scientific Reports"},{"issue":"45","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"28422","DOI":"10.1074\/jbc.271.45.28422","article-title":"Structure\u2010Function Relationships of the \n                     Saccharomyces cerevisiae\n                   Fatty Acid Synthase: Three\u2010Dimensional Structure","volume":"271","author":"Kolodziej S. J.","year":"1996","journal-title":"Journal of Biological Chemistry"},{"issue":"3","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0167-4838(82)90441-1","article-title":"Structure of Bacterial Fatty Acid Synthetase From \n                     Brevibacterium ammoniagenes","volume":"708","author":"Morishima N.","year":"1982","journal-title":"Biochimica et Biophysica Acta (BBA) \u2010 Protein Structure and Molecular Enzymology"},{"issue":"1","key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"3886","DOI":"10.1038\/s41467-018-06440-6","article-title":"Structure of Type\u2010I \n                     Mycobacterium tuberculosis\n                   Fatty Acid Synthase at 3.3 \u00c5 Resolution","volume":"9","author":"Elad N.","year":"2018","journal-title":"Nature Communications"},{"issue":"9","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"e0204457","DOI":"10.1371\/journal.pone.0204457","article-title":"Expression of a Recombinant, 4\u2032\u2010Phosphopantetheinylated, Active \n                     M. tuberculosis\n                   Fatty Acid Synthase I in \n                     E. coli","volume":"13","author":"Baron S.","year":"2018","journal-title":"PLoS One"},{"issue":"20","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"9164","DOI":"10.1073\/pnas.0913547107","article-title":"Direct Structural Insight Into the Substrate\u2010Shuttling Mechanism of Yeast Fatty Acid Synthase by Electron Cryomicroscopy","volume":"107","author":"Gipson P.","year":"2010","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"35","key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"12357","DOI":"10.1021\/ja103354w","article-title":"Mechanism of Substrate Shuttling by the Acyl\u2010Carrier Protein Within the Fatty Acid Mega\u2010Synthase","volume":"132","author":"Anselmi C.","year":"2010","journal-title":"Journal of the American Chemical Society"},{"issue":"1","key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1146\/annurev.micro.55.1.305","article-title":"Bacterial Fatty Acid Biosynthesis: Targets for Antibacterial Drug Discovery","volume":"55","author":"Campbell J. W.","year":"2001","journal-title":"Annual Review of Microbiology"},{"issue":"1","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1146\/annurev.biochem.74.082803.133524","article-title":"The Structural Biology of Type II Fatty Acid Biosynthesis","volume":"74","author":"White S. W.","year":"2005","journal-title":"Annual Review of Biochemistry"},{"issue":"1","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1432-1033.1977.tb11795.x","article-title":"Reaction of Yeast Fatty Acid Synthetase With Iodoacetamide: 1. Kinetics of Inactivation and Extent of Carboxamidomethylation","volume":"79","author":"Oesterhelt D.","year":"1977","journal-title":"European Journal of Biochemistry"},{"issue":"23","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"6598","DOI":"10.1021\/bi00344a044","article-title":"Yeast Fatty Acid Synthase: Structure to Function Relationship","volume":"24","author":"Singh N.","year":"1985","journal-title":"Biochemistry"},{"issue":"18","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1002\/cbic.201800809","article-title":"Fatty Acid Biosynthesis: Chain\u2010Length Regulation and Control","volume":"20","author":"Heil C. S.","year":"2019","journal-title":"ChemBioChem"},{"issue":"82","key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"11606","DOI":"10.1039\/C8CC06838K","article-title":"Analysis and Engineering of Substrate Shuttling by the Acyl Carrier Protein (ACP) in Fatty Acid Synthases (FASs)","volume":"54","author":"Rossini E.","year":"2018","journal-title":"Chemical Communications"},{"issue":"1","key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"e70412","DOI":"10.1002\/pro.70412","article-title":"Structural Basis of Product Recognition by \n                     Mycobacterium tuberculosis\n                   Fatty Acid Synthase","volume":"35","author":"Samani E. K.","year":"2026","journal-title":"Protein Science"},{"issue":"5822","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1126\/science.1138248","article-title":"Structure of Fungal Fatty Acid Synthase and Implications for Iterative Substrate Shuttling","volume":"316","author":"Jenni S.","year":"2007","journal-title":"Science"},{"issue":"5822","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1126\/science.1138249","article-title":"Structural Basis for Substrate Delivery by Acyl Carrier Protein in the Yeast Fatty Acid Synthase","volume":"316","author":"Leibundgut M.","year":"2007","journal-title":"Science"},{"issue":"35","key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"12803","DOI":"10.1073\/pnas.0805827105","article-title":"Inhibition of the Fungal Fatty Acid Synthase Type I Multienzyme Complex","volume":"105","author":"Johansson P.","year":"2008","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"1","key":"e_1_2_10_19_1","first-page":"1","article-title":"Steric Occlusion Regulates Proximal Interactions of Acyl Carrier Protein Domain in Fungal Fatty Acid Synthase","volume":"3","author":"Lou J. W.","year":"2020","journal-title":"Communications Biology"},{"issue":"12","key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1093\/jmcb\/mjz096","article-title":"Modulation of Fatty Acid Synthase by ATR Checkpoint Kinase Rad3","volume":"11","author":"Qiu S.","year":"2019","journal-title":"Journal of Molecular Cell Biology"},{"issue":"23","key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"5054","DOI":"10.1016\/j.cell.2023.10.009","article-title":"Reconstruction of a Fatty Acid Synthesis Cycle From Acyl Carrier Protein and Cofactor Structural Snapshots","volume":"186","author":"Singh K.","year":"2023","journal-title":"Cell"},{"issue":"5","key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1039\/b110221b","article-title":"The Claisen Condensation in Biology","volume":"19","author":"Heath R. J.","year":"2002","journal-title":"Natural Product Reports"},{"issue":"3","key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1002\/prot.21016","article-title":"Solution Structure of Acyl Carrier Protein From \n                     Nitrosomonas europaea","volume":"64","author":"Srisailam S.","year":"2006","journal-title":"Proteins: Structure, Function, and Bioinformatics"},{"issue":"12","key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1021\/ci800297v","article-title":"Mechanical Features of Plasmodium Falciparum Acyl Carrier Protein in the Delivery of Substrates","volume":"48","author":"Colizzi F.","year":"2008","journal-title":"Journal of Chemical Information and Modeling"},{"issue":"7483","key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nature12810","article-title":"Trapping the Dynamic Acyl Carrier Protein in Fatty Acid Biosynthesis","volume":"505","author":"Nguyen C.","year":"2014","journal-title":"Nature"},{"issue":"15","key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1021\/acs.jpclett.6b01230","article-title":"Atomic\u2010Level Characterization of the Chain\u2010Flipping Mechanism in Fatty\u2010Acids Biosynthesis","volume":"7","author":"Colizzi F.","year":"2016","journal-title":"Journal of Physical Chemistry Letters"},{"issue":"32","key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"11240","DOI":"10.1021\/ja505442h","article-title":"Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy","volume":"136","author":"Johnson M. N. R.","year":"2014","journal-title":"Journal of the American Chemical Society"},{"issue":"8","key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1042\/BCJ20160041","article-title":"Sticky Swinging Arm Dynamics: Studies of an Acyl Carrier Protein Domain From the Mycolactone Polyketide Synthase","volume":"473","author":"Vance S.","year":"2016","journal-title":"Biochemical Journal"},{"issue":"12","key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"6787","DOI":"10.1021\/acscatal.1c00745","article-title":"Structure and Mechanistic Analyses of the Gating Mechanism of Elongating Ketosynthases","volume":"11","author":"Mindrebo J. T.","year":"2021","journal-title":"ACS Catalysis"},{"issue":"1","key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1038\/s41467-020-15455-x","article-title":"Gating Mechanism of Elongating \u03b2\u2010Ketoacyl\u2010ACP Synthases","volume":"11","author":"Mindrebo J. T.","year":"2020","journal-title":"Nature Communications"},{"issue":"1","key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/prot.25765","article-title":"Structural Characterization of \u03b2\u2010Ketoacyl ACP Synthase I Bound to Platencin and Fragment Screening Molecules at Two Substrate Binding Sites","volume":"88","author":"Patterson E. I.","year":"2020","journal-title":"Proteins: Structure, Function, and Bioinformatics"},{"issue":"1","key":"e_1_2_10_32_1","first-page":"1","article-title":"Electron Cryomicroscopy Observation of Acyl Carrier Protein Translocation in Type I Fungal Fatty Acid Synthase","volume":"9","author":"Lou J. W.","year":"2019","journal-title":"Scientific Reports"},{"issue":"15","key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"168967","DOI":"10.1016\/j.jmb.2025.168967","article-title":"AlphaFold Protein Structure Database and 3D\u2010Beacons: New Data and Capabilities","volume":"437","author":"Fleming J.","year":"2025","journal-title":"Journal of Molecular Biology"},{"issue":"4","key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1021\/acs.jcim.3c01873","article-title":"Peripheral Linker Mediates Acyl Carrier Protein's Recognition of Dehydratase and Stabilizes Type\u2010I \n                     Mycobacterium tuberculosis\n                   Fatty Acid Synthase","volume":"64","author":"Kumar A.","year":"2024","journal-title":"Journal of Chemical Information and Modeling"},{"issue":"3","key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"100014","DOI":"10.1016\/j.crmeth.2021.100014","article-title":"Folding Non\u2010Homologous Proteins by Coupling Deep\u2010Learning Contact Maps With I\u2010TASSER Assembly Simulations","volume":"1","author":"Zheng W.","year":"2021","journal-title":"Cell Reports Methods"},{"issue":"8062","key":"e_1_2_10_36_1","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/s41586-025-08782-w","article-title":"Structural Dynamics of Human Fatty Acid Synthase in the Condensing Cycle","volume":"641","author":"Choi W.","year":"2025","journal-title":"Nature"},{"issue":"11","key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1002\/jcc.20945","article-title":"CHARMM\u2010GUI: A Web\u2010Based Graphical User Interface for CHARMM","volume":"29","author":"Jo S.","year":"2008","journal-title":"Journal of Computational Chemistry"},{"issue":"9","key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1021\/ct300400x","article-title":"Optimization of the Additive CHARMM All\u2010Atom Protein Force Field Targeting Improved Sampling of the Backbone \u03d5, \u03c8 and Side\u2010Chain \u03c7\n                  1 and \u03c7\n                  2 Dihedral Angles","volume":"8","author":"Best R. B.","year":"2012","journal-title":"Journal of Chemical Theory and Computation"},{"issue":"25","key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1002\/jcc.23354","article-title":"CHARMM36 All\u2010Atom Additive Protein Force Field: Validation Based on Comparison to NMR Data","volume":"34","author":"Huang J.","year":"2013","journal-title":"Journal of Computational Chemistry"},{"issue":"2","key":"e_1_2_10_40_1","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of Simple Potential Functions for Simulating Liquid Water","volume":"79","author":"Jorgensen W. L.","year":"1983","journal-title":"Journal of Chemical Physics"},{"issue":"1","key":"e_1_2_10_41_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1021\/acs.jctc.5b00935","article-title":"CHARMM\u2010GUI Input Generator for NAMD, GROMACS, AMBER, OpenMM, and CHARMM\/OpenMM Simulations Using the CHARMM36 Additive Force Field","volume":"12","author":"Lee J.","year":"2016","journal-title":"Journal of Chemical Theory and Computation"},{"issue":"4","key":"e_1_2_10_42_1","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/jcc.21367","article-title":"CHARMM General Force Field: A Force Field for Drug\u2010Like Molecules Compatible With the CHARMM All\u2010Atom Additive Biological Force Fields","volume":"31","author":"Vanommeslaeghe K.","year":"2010","journal-title":"Journal of Computational Chemistry"},{"issue":"31","key":"e_1_2_10_43_1","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1002\/jcc.23067","article-title":"Extension of the CHARMM General Force Field to Sulfonyl\u2010Containing Compounds and Its Utility in Biomolecular Simulations","volume":"33","author":"Yu W.","year":"2012","journal-title":"Journal of Computational Chemistry"},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High Performance Molecular Simulations Through Multi\u2010Level Parallelism From Laptops to Supercomputers","volume":"1","author":"Abraham M. J.","year":"2015","journal-title":"SoftwareX"},{"issue":"5","key":"e_1_2_10_45_1","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.jmgm.2010.10.007","article-title":"POVME: An Algorithm for Measuring Binding\u2010Pocket Volumes","volume":"29","author":"Durrant J. D.","year":"2011","journal-title":"Journal of Molecular Graphics & Modelling"},{"issue":"3","key":"e_1_2_10_46_1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1002\/prot.10421","article-title":"A Template Search Reveals Mechanistic Similarities and Differences in \u03b2\u2010Ketoacyl Synthases (KAS) and Related Enzymes","volume":"52","author":"Dawe J. H.","year":"2003","journal-title":"Proteins: Structure, Function, and Bioinformatics"},{"issue":"2","key":"e_1_2_10_47_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.cell.2007.03.013","article-title":"The Crystal Structure of Yeast Fatty Acid Synthase, a Cellular Machine With Eight Active Sites Working Together","volume":"129","author":"Lomakin I. B.","year":"2007","journal-title":"Cell"},{"issue":"2","key":"e_1_2_10_48_1","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1107\/S2052252519017366","article-title":"The Resolution Revolution in cryoEM Requires High\u2010Quality Sample Preparation: A Rapid Pipeline to a High\u2010Resolution Map of Yeast Fatty Acid Synthase","volume":"7","author":"Joppe M.","year":"2020","journal-title":"IUCrJ"},{"issue":"44","key":"e_1_2_10_49_1","doi-asserted-by":"crossref","first-page":"17874","DOI":"10.1073\/pnas.1311599110","article-title":"Native Contacts Determine Protein Folding Mechanisms in Atomistic Simulations","volume":"110","author":"Best R. B.","year":"2013","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"13","key":"e_1_2_10_50_1","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1021\/bi5014563","article-title":"Interactions of the Acyl Chain With the \n                     Saccharomyces cerevisiae\n                   Acyl Carrier Protein","volume":"54","author":"Perez D. R.","year":"2015","journal-title":"Biochemistry"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1101\/2024.07.17.603935"},{"issue":"15","key":"e_1_2_10_52_1","doi-asserted-by":"crossref","first-page":"9502","DOI":"10.1021\/acs.chemrev.1c00147","article-title":"Animal Fatty Acid Synthase: A Chemical Nanofactory","volume":"121","author":"Paiva P.","year":"2021","journal-title":"Chemical Reviews"},{"issue":"1","key":"e_1_2_10_53_1","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1074\/jbc.M703454200","article-title":"A Mammalian Type I Fatty Acid Synthase Acyl Carrier Protein Domain Does Not Sequester Acyl Chains","volume":"283","author":"Ploskon E.","year":"2008","journal-title":"Journal of Biological Chemistry"}],"container-title":["Proteins: Structure, Function, and Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prot.70193","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prot.70193","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prot.70193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:05:28Z","timestamp":1792634728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prot.70193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,21]]},"references-count":52,"alternative-id":["10.1002\/prot.70193"],"URL":"https:\/\/doi.org\/10.1002\/prot.70193","archive":["Portico"],"relation":{},"ISSN":["0887-3585","1097-0134"],"issn-type":[{"value":"0887-3585","type":"print"},{"value":"1097-0134","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,21]]},"assertion":[{"value":"2026-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"prot.70193"}}