{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:50:02Z","timestamp":1780638602052,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T00:00:00Z","timestamp":1670716800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007207","name":"Toray Industries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007207","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The innate immune system has an emerging role as a mediator of neuro\u2010immune communication and a therapeutic target for itch. Toll\u2010like receptor 3 (TLR3) plays an important role in itch, as shown in TLR3 knock\u2010out mice. In this study, to evaluate effects of TLR3 inhibitors on histamine\u2010independent itch, we used two kinds of isothiocyanate (ITC). Both phenethyl isothiocyanate (PEITC) and sulforaphane (SFN) inhibited Poly I:C (PIC)\u2010induced signaling in the RAW264.7 cell line. We then investigated the anti\u2010pruritic effect of these compounds on PIC\u2010 and chloroquine (CQ)\u2010induced scratching behavior. PEITC and SFN both suppressed PIC\u2010evoked scratching behavior in mice, and PEITC also inhibited CQ\u2010induced acute itch. Finally, we examined the oxazolone\u2010induced chronic itch model in mice. Surprisingly, oral dosing of both compounds suppressed scratching behaviors that were observed in mice. Our findings demonstrate that TLR3 is a critical mediator in acute and chronic itch transduction in mice and may be a promising therapeutic target for pruritus in human skin disorders. It is noteworthy that SFN has potential for use as an antipruritic as it is a phytochemical that is used as a supplement.<\/jats:p>","DOI":"10.1002\/prp2.1038","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T02:04:40Z","timestamp":1670810680000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010pruritic effect of isothiocyanates: Potential involvement of toll\u2010like receptor 3 signaling"],"prefix":"10.1002","volume":"10","author":[{"given":"Masaki","family":"Moriyama","sequence":"first","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1396-455X","authenticated-orcid":false,"given":"Mitsuhiro","family":"Konno","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Serizawa","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsumi","family":"Yuzawa","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Majima","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuo","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mie","family":"Kainoh","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories Toray Industries, Inc.  Kamakura Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0305"},{"key":"e_1_2_12_3_1","first-page":"35","article-title":"Atopic dermatitis: an overview","volume":"86","author":"Berke R","year":"2012","journal-title":"Am Fam Physician"},{"key":"e_1_2_12_4_1","first-page":"173","article-title":"Atopic dermatitis prevention and treatment","volume":"100","author":"Silverberg NB","year":"2017","journal-title":"Cutis"},{"key":"e_1_2_12_5_1","first-page":"1","article-title":"PatientsLikeMe and atopic dermatitis: characterizing the atopic dermatitis patient profile","volume":"24","author":"William Rundle C","year":"2018","journal-title":"Dermatol Online J"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.03.023"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.12.022"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.7573\/dic.2020-8-5"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1917006"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2018.10.001"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.09.005"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.04.018"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.03.007"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2019.05.005"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45414"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.02.012"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202093"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10050580"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25030575"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.591802"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228637"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3912"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)01828-4"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1121"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14748"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.55.195"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4290-06.2007"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511400225"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500108"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809853115"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-3343"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.retram.2016.08.006"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001551"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3881"}],"container-title":["Pharmacology Research &amp; Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.1038","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prp2.1038","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.1038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T17:01:56Z","timestamp":1692550916000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/prp2.1038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/prp2.1038"],"URL":"https:\/\/doi.org\/10.1002\/prp2.1038","archive":["Portico"],"relation":{},"ISSN":["2052-1707","2052-1707"],"issn-type":[{"value":"2052-1707","type":"print"},{"value":"2052-1707","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"2022-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01038"}}