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Pharmacological functional MRI (phfMRI) has been used to evaluate regional brain hemodynamic changes induced by neurovascular coupling resulting from pharmacological interventions, thus phfMRI studies can be used to help understand the neurophysiological function of specific receptors. Herein, the blood oxygenation level\u2010dependent (BOLD) signal changes associated with D1R action in anesthetized rats was investigated by using a preclinical ultra\u2010high\u2010field 11.7\u2010T MRI scanner. PhfMRI was performed before and after administration of the D1\u2010like receptor agonist (SKF82958), antagonist (SCH39166), or physiological saline subcutaneously. Compared to saline, the D1\u2010agonist induced a BOLD signal increase in the striatum, thalamus, prefrontal cortex, and cerebellum. At the same time, the D1\u2010antagonist reduced the BOLD signal in the striatum, thalamus, and cerebellum by evaluating temporal profiles. PhfMRI detected D1R\u2010related BOLD signal changes in the brain regions associated with high expression of D1R. We also measured the early expression of c\u2010fos at the mRNA level to evaluate the effects of SKF82958 and isoflurane anesthesia on neuronal activity. Regardless of the presence of isoflurane anesthesia, c\u2010fos expression level was increased in the region where positive BOLD responses were observed with administration of SKF82958. These findings demonstrated that phfMRI could be used to identify the effects of direct D1 blockade on physiological brain functions and also for neurophysiological assessment of dopamine receptor functions in living animals.<\/jats:p>","DOI":"10.1002\/prp2.1055","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T06:30:08Z","timestamp":1676874608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultra\u2010high\u2010field pharmacological functional <scp>MRI<\/scp> of dopamine <scp>D1<\/scp> receptor\u2010related interventions in anesthetized rats"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9741-3159","authenticated-orcid":false,"given":"Yuka","family":"Kimura","sequence":"first","affiliation":[{"name":"Drug Development Research Laboratories Sumitomo Dainippon Pharma Co Ltd  Osaka Japan"},{"name":"Graduate School of Science and 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