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The aim of the present study is to investigate the anti\u2010tumor activity of cannabinoids, CBD (cannbidiol), and CBG (cannabigerol) in ovarian carcinoma cells sensitive and resistant to chemotherapeutic drugs. Sensitive A2780 cells and resistant A2780\/CP70 carcinoma cells and non\u2010carcinoma cells were exposed to varying concentrations of CBD, CBG, carboplatin or CB<jats:sub>1<\/jats:sub> and CB<jats:sub>2<\/jats:sub> receptor antagonists, AM251 and AM630, respectively, alone or in combination, at different exposure times and cytotoxicity was measured by MTT assay. The mechanism of action of CBD and CB in inducing cytotoxicity was investigated involving a variety of apoptotic and cell cycle assays. Treatment with CBD and CBG selectively, dose and time dependently reduced cell viability and induced apoptosis. The effect of CBD was stronger than CBG in all cell lines tested. Both CBD and CBG induced stronger cytotoxicity than afforded by carboplatin in resistant cells. The cytotoxicity induced by CBD was not CB<jats:sub>1<\/jats:sub> or CB<jats:sub>2<\/jats:sub> receptor dependent in both carcinoma cells, however, CBG\u2010induced cytotoxicity may involve CB<jats:sub>1<\/jats:sub> receptor activity in cisplatin\u2010resistant carcinoma cells. A synergistic effect was observed when cannabinoids at sublethal doses were combined with carboplatin in both carcinoma cells. The apoptotic event may involve loss of mitochondrial membrane potential, Annexin V, caspase 3\/7, ROS activities, and cell cycle arrest. Further studies are required to investigate whether these results are translatable in the clinic. Combination therapies with conventional cancer treatments using cannabinoids are suggested.<\/jats:p>","DOI":"10.1002\/prp2.1152","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T20:09:09Z","timestamp":1702670949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigation of the cytotoxicity induced by cannabinoids on human ovarian carcinoma cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Kartheek","family":"Sooda","sequence":"first","affiliation":[{"name":"Department of Pharmacy, School of Applied Sciences University of Huddersfield  Huddersfield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon J.","family":"Allison","sequence":"additional","affiliation":[{"name":"Department of Biological &amp; Geographical Sciences, School of Applied Sciences University of Huddersfield  Huddersfield 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