{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:06:58Z","timestamp":1753870018626,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T00:00:00Z","timestamp":1707523200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the functional role of phosphodiesterase enzymes (PDE) in the isolated porcine ureter. Distal ureteral strips were mounted in organ baths and pre\u2010contracted with 5\u2010HT (100\u2009\u03bcM). Upon generation of stable phasic contractions, PDE\u20104 and PDE\u20105 inhibitors were added cumulatively to separate tissues. PDE\u20104 inhibitors, such as rolipram (10\u2009nM and greater) and roflumilast (100\u2009nM and greater), resulted in significant attenuation of ureteral contractile responses, while a higher concentration of piclamilast (1\u2009\u03bcM and greater) was required to induce a significant depressant effect. The attenuation effect by rolipram was abolished by SQ22536 (100\u2009\u03bcM). PDE\u20105 inhibitors, such as sildenafil and tadalafil, were not nearly as effective and were only able to suppress the 5\u2010HT\u2010induced contractions at higher concentrations of 1\u2009\u03bcM. Rolipram significantly enhanced the depressant effect of forskolin, while sodium nitroprusside\u2010induced attenuation of contractile responses remained unchanged in the presence of tadalafil. In summary, our study demonstrates that PDE\u20104 inhibitors are effective in attenuating 5\u2010HT\u2010induced contractility in porcine distal ureteral tissues, while PDE\u20105 inhibitors are less effective. These findings suggest that PDE\u20104 inhibitors, such as rolipram, may hold promise as potential therapeutic agents for the treatment of ureteral disorders attributable to increased intra\u2010ureteral pressure.<\/jats:p>","DOI":"10.1002\/prp2.1175","type":"journal-article","created":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T09:04:25Z","timestamp":1707555865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of <scp>PDE<\/scp>\u20104 isoenzyme attenuates frequency and overall contractility of agonist\u2010evoked ureteral phasic contractions"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5866-8968","authenticated-orcid":false,"given":"Iris","family":"Lim","sequence":"first","affiliation":[{"name":"Centre for Urology, Faculty of Health Sciences &amp; Medicine Bond University  Gold Coast Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taishi","family":"Masutani","sequence":"additional","affiliation":[{"name":"Department of Cell Physiology Nagoya City University Graduate School of Medical Sciences  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Hashitani","sequence":"additional","affiliation":[{"name":"Department of Cell Physiology Nagoya City University Graduate School of Medical Sciences  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russ","family":"Chess\u2010Williams","sequence":"additional","affiliation":[{"name":"Centre for Urology, Faculty of Health Sciences &amp; 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