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Current treatments for arthritis, including biological disease\u2010modifying antirheumatic drugs, are not well\u2010tolerated in a substantial subset of arthritis patients and are expensive; therefore, new treatments are needed. Pharmacokinetic parameters of ELP\u2010004 were tested with intravenous, oral, and subcutaneous administration and found to be rapidly absorbed and distributed. We found that ELP\u2010004 was non\u2010mutagenic, did not induce chromosome aberrations, non\u2010cardiotoxic, and had minimal off\u2010target effects. Using in\u00a0vitro hepatic systems, we found that ELP\u2010004 is primarily metabolized by CYP1A2 and CYP2B6 and predicted metabolic pathways were identified. Finally, we show that ELP\u2010004 inhibits osteoclast differentiation without suppressing overall T\u2010cell function. These preclinical data will inform future development of an oral compound as well as in\u00a0vivo efficacy studies in mice.<\/jats:p>","DOI":"10.1002\/prp2.1230","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T11:41:37Z","timestamp":1719574897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preclinical evaluation of ELP\u2010004 in mice"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5914-6336","authenticated-orcid":false,"given":"Jamie L.","family":"McCall","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology West Virginia University School of Medicine  Morgantown West Virginia USA"},{"name":"ExesaLibero Pharma, Inc.  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