{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:38:10Z","timestamp":1790735890970,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007247","name":"Svenska Kulturfonden","doi-asserted-by":"publisher","award":["187699"],"award-info":[{"award-number":["187699"]}],"id":[{"id":"10.13039\/501100007247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014438","name":"Business Finland","doi-asserted-by":"publisher","award":["253\/31\/2019"],"award-info":[{"award-number":["253\/31\/2019"]}],"id":[{"id":"10.13039\/501100014438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014438","name":"Business Finland","doi-asserted-by":"publisher","award":["473\/31\/2015"],"award-info":[{"award-number":["473\/31\/2015"]}],"id":[{"id":"10.13039\/501100014438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010112","name":"Victoriastiftelsen","doi-asserted-by":"publisher","award":["20240472"],"award-info":[{"award-number":["20240472"]}],"id":[{"id":"10.13039\/100010112","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["315139"],"award-info":[{"award-number":["315139"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["332582"],"award-info":[{"award-number":["332582"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21OC0068904"],"award-info":[{"award-number":["NNF21OC0068904"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The association of hormonal contraception with increased risk of inflammatory bowel disease (IBD) observed in females suggests involvement of ovarian hormones, such as estradiol, and the estrogen receptors in the progression of intestinal inflammation. Here, we investigated the effects of prophylactic SERM2 and estradiol supplementation in dextran sulfate sodium\u2010induced colitis using mice with intact ovaries and ovariectomized (OVX) female mice. We found that graded colitis score was threefold reduced in the OVX mice, compared to mice with intact ovaries. Estradiol supplementation, however, aggravated the colitis in OVX mice, increasing the colitis score to a similar level than what was observed in the intact mice. Further, we observed that immune infiltration and gene expression of inflammatory interleukins <jats:italic>Il1b<\/jats:italic>, <jats:italic>Il6<\/jats:italic>, and <jats:italic>Il17a<\/jats:italic> were up to 200\u2010fold increased in estradiol supplemented OVX colitis mice, while a mild but consistent decrease was observed by SERM2 treatment in intact animals. Additionally, cyclo\u2010oxygenase 2 induction was increased in the colon of colitis mice, in correlation with increased serum estradiol levels. Measured antagonist properties of SERM2, together with the other results presented here, indicates an exaggerating role of ER\u03b1 signaling in colitis. Our results contribute to the knowledge of ovarian hormone effects in colitis and encourage further research on the potential use of ER antagonists in the colon, in order to alleviate inflammation.<\/jats:p>","DOI":"10.1002\/prp2.1234","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T04:29:50Z","timestamp":1720067390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Estrogen deprivation and estrogen receptor \u03b1 antagonism decrease <scp>DSS<\/scp> colitis in female mice"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8039-8535","authenticated-orcid":false,"given":"Anja","family":"Hjelt","sequence":"first","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santeri","family":"Anttila","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4598-2450","authenticated-orcid":false,"given":"Anu","family":"Wiklund","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1482-9154","authenticated-orcid":false,"given":"Anne","family":"Rokka","sequence":"additional","affiliation":[{"name":"Turku Bioscience University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darin","family":"Al\u2010Ramahi","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"},{"name":"Bioanalytical Laboratory University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7165-9839","authenticated-orcid":false,"given":"Diana M.","family":"Toivola","sequence":"additional","affiliation":[{"name":"Faculty of Science and Engineering, Department of Biosciences, Cell Biology \u00c5bo Akademi University  Turku Finland"},{"name":"InFLAMES Research Flagship Center \u00c5bo Akademi University  Turku Finland"},{"name":"Turku Centre for Disease Modeling University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0052-6489","authenticated-orcid":false,"given":"Lauri","family":"Polari","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"},{"name":"Faculty of Science and Engineering, Department of Biosciences, Cell Biology \u00c5bo Akademi University  Turku Finland"},{"name":"InFLAMES Research Flagship Center \u00c5bo Akademi University  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8752-6862","authenticated-orcid":false,"given":"Jorma","family":"M\u00e4\u00e4tt\u00e4","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Turku  Turku Finland"},{"name":"Turku Centre for Disease Modeling University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2002697"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-00360-x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2007-0001"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2022-065186"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/J.GASTRO.2018.06.043"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000000915"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.16647"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2015.01.018"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IMMUNI.2023.10.008"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/ONCOTARGET.6217"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10620-017-4717-5\/FIGURES\/4"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.STEROIDS.2018.09.013"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS20133175"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-015-0180-7"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.5009\/GNL18221"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/GUTJNL-2012-304216"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.7980"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCMGH.2017.12.003"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JNUTBIO.2020.108438"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022219"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.0405841102\/SUPPL_FILE\/05841TABLE3.HTML"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/S40169-017-0157-2"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/GLIA.20805"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE.1.06063"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91320-1"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.21.2.255"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS23169344"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0763-1"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20123007"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.50.4.507"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKX1121"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/BPH.16179"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CANLET.2020.06.021"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2022.111454"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/SJ.ONC.1203828"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/ME.2002-0206"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.97.11.5936"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2003.11.008"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199308053290607"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/JIMMUNOL.175.8.5024"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS19051442"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2020.01315\/BIBTEX"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1615"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.09.076"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/JBC.M800524200"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/CARCIN\/BGQ268"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(97)00193-7"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365-2826.1994.TB00626.X"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1095\/BIOLREPROD.116.139881\/2863883"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CROHNS.2011.11.004"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.7759\/CUREUS.11027"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64789-X"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1053\/J.GASTRO.2012.07.096"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.120.000216"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-023-00822-7"}],"container-title":["Pharmacology Research &amp; Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.1234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T03:29:13Z","timestamp":1723778953000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/prp2.1234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,3]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/prp2.1234"],"URL":"https:\/\/doi.org\/10.1002\/prp2.1234","archive":["Portico"],"relation":{},"ISSN":["2052-1707","2052-1707"],"issn-type":[{"value":"2052-1707","type":"print"},{"value":"2052-1707","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,3]]},"assertion":[{"value":"2024-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1234"}}