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Elevated levels of<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>have been associated with painful pathologies such as diabetic neuropathy and fibromyalgia. However, completely inhibiting the<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>signal could hold significant side effects, such as those observed in a genetic condition called congenital insensitivity to pain and anhidrosis (<jats:styled-content style=\"fixed-case\">CIPA<\/jats:styled-content>). Previous methods of screening for<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010inhibitors used labeling techniques which have the potential to alter molecular interactions.<jats:styled-content style=\"fixed-case\">SPR<\/jats:styled-content>spectroscopy and<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010dependent cellular assays were utilized to identify a novel<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010inhibitor,<jats:styled-content style=\"fixed-case\">BVNP<\/jats:styled-content>\u20100197 (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a090\u00a0nmol\/L), the first<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010inhibitor described with a high nanomolar<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>inhibition efficiency. The present study utilizes molecular modeling flexible docking to identify a novel binding domain in the loop<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>cleft of<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/prp2.339","type":"journal-article","created":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T00:44:57Z","timestamp":1504313097000},"source":"Crossref","is-referenced-by-count":8,"title":["Nerve growth factor inhibitor with novel\u2010binding domain demonstrates nanomolar efficacy in both cell\u2010based and cell\u2010free assay systems"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0247-0626","authenticated-orcid":false,"given":"Allison E.","family":"Kennedy","sequence":"first","affiliation":[{"name":"Northern Ontario School of Medicine Sudbury Ontario Canada"},{"name":"Laurentian University Biomolecular Sciences Program Sudbury Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey A.","family":"Laamanen","sequence":"additional","affiliation":[{"name":"Northern Ontario School of Medicine Sudbury Ontario Canada"},{"name":"Laurentian University Bharti School of Engineering Sudbury Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell S.","family":"Ross","sequence":"additional","affiliation":[{"name":"Northern Ontario School of Medicine Sudbury Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Vohra","sequence":"additional","affiliation":[{"name":"Northern Ontario School of Medicine Sudbury Ontario Canada"},{"name":"Sussex Research Laboratories Inc. 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