{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:07:02Z","timestamp":1753870022667,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001771","name":"University of Newcastle Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001771","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP150103231"],"award-info":[{"award-number":["DP150103231"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There remains a compelling need for the development of nonsurgical sterilizing agents to expand the fertility management options for both domestic and feral animal species. We hypothesize that an efficacious sterilization approach would be to selectively ablate nonrenewable cell types that are essential for reproduction, such as the undifferentiated gonocytes within the embryonic gonad. Here, we report a novel strategy to achieve this goal centered on the use of a chemically modified M13 bacteriophage to effect the targeted delivery of menadione, a redox\u2010cycling naphthoquinone, to mouse gonocytes. Panning of the M13 random peptide \u2018phage display library proved effective in the isolation of gonocyte\u2010specific targeting clones. One such clone was modified via N\u2010succinimidyl\u2010S\u2010acetylthioacetate (SATA) linkage to the N\u2010terminus of the major PVIII capsid protein. Subsequent deacetylation of the SATA was undertaken to expose a thiol group capable of reacting with menadione through Michael addition. This chemical modification was confirmed using UV spectrophotometry. In proof\u2010of\u2010concept experiments we applied the modified \u2018phage to primary cultures of fetal germ cells and induced, an approximately, 60% reduction in the viability of the target cell population. These studies pave the way for in vivo application of chemically modified M13 bacteriophage in order to achieve the selective ablation of nonrenewable cell types in the reproductive system, thereby providing a novel nonsurgical approach the regulation of fertility in target species.<\/jats:p>","DOI":"10.1002\/prp2.654","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T15:44:05Z","timestamp":1600962245000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel approach to nonsurgical sterilization; application of menadione\u2010modified gonocyte\u2010targeting M13 bacteriophage for germ cell ablation <i>in utero<\/i>"],"prefix":"10.1002","volume":"8","author":[{"given":"Barbara A.","family":"Fraser","sequence":"first","affiliation":[{"name":"Priority Research Centre for Reproductive Science The University of Newcastle Callaghan NSW Australia"},{"name":"Pregnancy and Reproduction Program Hunter Medical Research Institute New Lambton Heights NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasey","family":"Miller","sequence":"additional","affiliation":[{"name":"Priority Research Centre for Reproductive Science The University of Newcastle Callaghan NSW Australia"},{"name":"Pregnancy and Reproduction Program Hunter Medical Research Institute New Lambton Heights NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie A.","family":"Trigg","sequence":"additional","affiliation":[{"name":"Priority Research Centre for Reproductive Science The University of Newcastle Callaghan NSW Australia"},{"name":"Pregnancy and Reproduction Program Hunter Medical Research Institute New Lambton Heights NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan D.","family":"Smith","sequence":"additional","affiliation":[{"name":"Analytical and Biomolecular Research Facility The University of Newcastle Callaghan NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick S.","family":"Western","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Health Hudson Institute of Medical Research Clayton Victoria Australia"},{"name":"Department of Molecular and Translational Science Monash University Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brett","family":"Nixon","sequence":"additional","affiliation":[{"name":"Priority Research Centre for Reproductive Science The University of Newcastle Callaghan NSW Australia"},{"name":"Pregnancy and Reproduction Program Hunter Medical Research Institute New Lambton Heights NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9152-156X","authenticated-orcid":false,"given":"Robert J.","family":"Aitken","sequence":"additional","affiliation":[{"name":"Priority Research Centre for Reproductive Science The University of Newcastle Callaghan NSW Australia"},{"name":"Pregnancy and Reproduction Program Hunter Medical Research Institute New Lambton Heights NSW Australia"},{"name":"Faculty of Health and Medicine The University of Newcastle Callaghan NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.04.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.07.016"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.02079.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12862"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20142"},{"issue":"706","key":"e_1_2_9_7_1","article-title":"Changes in the Ovary of the Mouse following Exposure to X\u2010rays. Part I. \u2010 Irradiation at Three Weeks Old","volume":"101","author":"Brambell FW","year":"1927","journal-title":"Proc R Soc Lond B"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402052"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"issue":"12","key":"e_1_2_9_10_1","article-title":"Reactive oxygen species and sperm cells","volume":"2","author":"Sanocka D","year":"2004","journal-title":"Reproductive Biology and Endocrinology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4161\/oxim.1.1.6843"},{"issue":"3","key":"e_1_2_9_12_1","article-title":"Redox Regulation of Human Sperm function: From Physiological Control of Sperm Capacitation to the Etiology of Infertility and DNA Damage in the Germ Line","volume":"14","author":"Aitken RJ","year":"2011","journal-title":"Antoxidants and Redox Signaling"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox104"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.102020"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(91)90279-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1269\/jrr.09100"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/019262301301418892"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095224"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2889"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003999"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2008.12.004"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2711"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq272"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.4001944"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-016-0223-x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2013.09.001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16021211"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16021559"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00163-07"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2017.1410259"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc300130z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00734"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.09.011"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc900405q"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb300052u"},{"issue":"1","key":"e_1_2_9_36_1","first-page":"159","article-title":"Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis","volume":"96","author":"Zhou W","year":"2017","journal-title":"Biol Reprod"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-741-6:001"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-436-0_3"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.22.4585"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-5049-9"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01202a025"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2797(87)90003-2"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2011.09.003"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.09.003"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339511"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn362"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00343"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1259\/0007-1285-53-628-271"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02660"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.2144\/04371ST04"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.06.014"}],"container-title":["Pharmacology Research &amp; Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prp2.654","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.654","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T05:05:46Z","timestamp":1693890346000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/prp2.654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,15]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/prp2.654"],"URL":"https:\/\/doi.org\/10.1002\/prp2.654","archive":["Portico"],"relation":{},"ISSN":["2052-1707","2052-1707"],"issn-type":[{"type":"print","value":"2052-1707"},{"type":"electronic","value":"2052-1707"}],"subject":[],"published":{"date-parts":[[2020,9,15]]},"assertion":[{"value":"2020-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00654"}}