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Female Wistar rats were submitted to L5\/L6 spinal nerve ligation (SNL) or repeated doses of cisplatin (0.1\u00a0mg\/kg, i.p.) to induce experimental neuropathy. Then, tactile allodynia was determined, and animals were treated with logarithmic doses of PhAR\u2010DBH\u2010Me (3.2\u2010100\u00a0mg\/kg, i.p.). To evaluate the mechanism of action of PhAR\u2010DBH\u2010Me, in silico studies using crystallized structures of CB1, CB2, and TRPV1 receptors were performed. To corroborate the computational insights, animals were intraperitoneally administrated with antagonists for CB1 (AM\u2010251, 3\u00a0mg\/kg), CB2 (AM\u2010630, 1\u00a0mg\/kg), and TRPV1 receptors (capsazepine, 3\u00a0mg\/kg), 15\u00a0min before to PhAR\u2010DBH\u2010Me (100\u00a0mg\/kg) administration. Vagal stimulation evoked on striated muscle contraction in esophagus, was used to elicited pharmacological response of PhAR\u2010DBH\u2010ME on nervous tissue. Systemic administration of PhAR\u2010DBH\u2010Me reduced the SNL\u2010 and cisplatin\u2010induced allodynia. Docking studies suggested that PhAR\u2010DBH\u2010Me acts as an agonist for CB1, CB2, and TRPV1 receptors, with similar affinity to the endogenous ligand anandamide. Moreover antiallodynic effect of PhAR\u2010DBH\u2010Me was partially prevented by administration of AM\u2010251 and AM\u2010630, and completely prevented by capsazepine. Finally, PhAR\u2010DBH\u2010Me decreased the vagally evoked electrical response in esophagus rat. Taken together, results indicate that PhAR\u2010DBH\u2010Me induces an antiallodynic effect through partial activation of CB1 and CB2 receptors, as well as desensitization of TRPV1 receptors. Data also shed light on the novel vanilloid nature of the synthetic compound PhAR\u2010DBH\u2010Me.<\/jats:p>","DOI":"10.1002\/prp2.663","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T14:43:56Z","timestamp":1600872236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antiallodynic effect of PhAR\u2010DBH\u2010Me involves cannabinoid and TRPV1 receptors"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8006-3022","authenticated-orcid":false,"given":"Geovanna Nallely","family":"Qui\u00f1onez\u2010Bastidas","sequence":"first","affiliation":[{"name":"Facultad de Qu\u00edmica Departamento de Farmacia Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad Universitaria Coyoac\u00e1n Ciudad de M\u00e9xico 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