{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T21:51:59Z","timestamp":1787262719713,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Sirolimus is a third\u2010generation immunosuppressant with multiple efficacies, including antifungal, antitumor, and immunosuppressive properties. We conducted a pharmacokinetic study to determine the effect of berberine hydrochloride (BBR) on the pharmacokinetics of sirolimus in rats. Thirty\u2010six rats were randomly divided into six groups, and blood samples were collected from the posterior orbital venous plexus at different time points after the last administration to determine the plasma concentration of sirolimus. Liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS) was used for detection, and DAS 2.0 software was employed for pharmacokinetic analysis. A single administration of BBR significantly reduced the pharmacokinetic parameter AUC<jats:sub>0\u201012h<\/jats:sub> of sirolimus. Repeated administration of BBR resulted in an upward trend in <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> AUC<jats:sub>0\u201012h<\/jats:sub>, AUC<jats:sub>0\u2010\u221e<\/jats:sub>, and CLz all show an upward trend. This study indicates that BBR has a certain impact on the pharmacokinetics of sirolimus in rats. However, further validation of the role of gene polymorphism in pharmacokinetics and clinical efficacy is needed for further research in a wider population.<\/jats:p>","DOI":"10.1002\/prp2.70126","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T09:43:23Z","timestamp":1747820603000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Effects of Different Administration Regimens of Berberine Hydrochloride on the Pharmacokinetics of Sirolimus in Rats"],"prefix":"10.1002","volume":"13","author":[{"given":"Yuanxia","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacy People's Hospital of Longhua  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory People's Hospital of Longhua  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingdong","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology People's Hospital of Longhua  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2751-7343","authenticated-orcid":false,"given":"Liwan","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmacy People's Hospital of Longhua  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdi113"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767030-00004"},{"issue":"31","key":"e_1_2_12_4_1","first-page":"173","article-title":"Research Progress on Clinical Application and Drug Interactions of Sirolimus","volume":"11","author":"Wang W.","year":"2018","journal-title":"Journal of Clinical Rational Drug Use"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181613e65"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200645110-00007"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612827666210715155809"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2018.06.014"},{"key":"e_1_2_12_9_1","first-page":"1","article-title":"A Patent Review of CYP3A4 Inhibitors (2018 \u2010 Present)","volume":"24","author":"Tu D. Z.","year":"2025","journal-title":"Expert Opinion on Therapeutic Patents"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics12090882"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-023-03531-2"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498254.2023.2290648"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2017.03.024"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.121.000508"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.0c00182"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2013-z"},{"issue":"4","key":"e_1_2_12_17_1","first-page":"930","article-title":"Study on the Pharmacokinetics of Xianlingpi Granules In\u00a0Vivo Based on UPLC\u2010QQQ\u2010MS Technology","volume":"22","author":"Wang Y.","year":"2024","journal-title":"Journal of Central South Pharmacy"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-332"},{"issue":"4","key":"e_1_2_12_19_1","first-page":"1","article-title":"Research Progress on Cytochrome P450 Gene Polymorphism and Drug Metabolism","volume":"15","author":"Zhao Y.","year":"2017","journal-title":"Journal of Clinical Drug Therapy"},{"issue":"32","key":"e_1_2_12_20_1","first-page":"4604","article-title":"Research Progress in Monitoring Sirolimus Blood Concentration","volume":"26","author":"Xie P.","year":"2015","journal-title":"Chinese Pharmacy"},{"issue":"4","key":"e_1_2_12_21_1","first-page":"449","article-title":"Systematic Evaluation of the Effect of Drug Gene Polymorphism on Sirolimus Blood Concentration in Renal Transplant Recipients","volume":"41","author":"Ren X.","year":"2022","journal-title":"Journal of Medicine and Pharmacy"},{"key":"e_1_2_12_22_1","unstructured":"Q.Ding \u201cStudy on the Relationship Between CYP3A5*3 MDR1C3435T Gene Polymorphism and Sirolimus Blood Concentration in Healthy Volunteers \u201d2017Hebei North University."},{"issue":"31","key":"e_1_2_12_23_1","first-page":"5825","article-title":"Literature Analysis of Adverse Reactions Caused by Sirolimus After Renal Transplantation","volume":"14","author":"Huo W.","year":"2010","journal-title":"Chinese Journal of Tissue Engineering Research and Clinical Rehabilitation"},{"issue":"1","key":"e_1_2_12_24_1","first-page":"66","article-title":"Meta\u2010Analysis of CYP3A5 Gene Polymorphism and Sirolimus Pharmacokinetics in Renal Transplant Recipients","volume":"32","author":"Ding Q.","year":"2016","journal-title":"Journal of PLA Pharmaceutical Sciences"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21040464"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.5414\/CP202499"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0307286"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27671"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23082042"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.1423"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2023.2282180"}],"container-title":["Pharmacology Research &amp; Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.70126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T10:19:46Z","timestamp":1750155586000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/prp2.70126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,21]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/prp2.70126"],"URL":"https:\/\/doi.org\/10.1002\/prp2.70126","archive":["Portico"],"relation":{},"ISSN":["2052-1707","2052-1707"],"issn-type":[{"value":"2052-1707","type":"print"},{"value":"2052-1707","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,21]]},"assertion":[{"value":"2025-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70126"}}