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Elevated monoamine oxidase\u2010A (MAO\u2010A) mRNA expression and activity in tumorous prostate positively correlate with disease progression and therapy resistance. While MAO\u2010B mRNA expression was also demonstrated in PAC cell lines, its role remained unclear. Therefore, this study evaluates the effects of the irreversible MAO\u2010B inhibitor selegiline and rasagiline and their combinations with conventional therapies on androgen\u2010insensitive (PC\u20103, DU145) and androgen\u2010sensitive (22Rv1, LNCaP, VCaP) PAC cell lines. MAO activity was determined by the MAO\u2010Glo luminescence assay, viability by the ATP\u2010based chemiluminescence method, proliferation by the Luna\u2010II automated cell counter, and mRNA expressions by RT\u2010qPCR. MAO\u2010B mRNA was stably expressed by all PAC cell lines, with the highest expression in 22Rv1 and LNCaP cells. Selegiline reduced MAO\u2010B activity by 75%\u201380% and decreased cell counts by 40%\u201350% at 100\u2009\u03bcM in PC\u20103 and 22Rv1 cells. Selegiline concentration\u2010dependently inhibited cell proliferation (100\u2009\u03bcM\u201310\u2009mM) and reduced viability (1\u201310\u2009mM) similar to rasagiline in all cell lines. Combination with enzalutamide in 22Rv1, and with docetaxel in PC\u20103 demonstrated potentiating and additive effects, respectively. Selegiline reduced <jats:italic>FOXA1<\/jats:italic> and <jats:italic>GLUT1<\/jats:italic> mRNA expressions related to cancer progression and metabolism in both cell lines, increased the apoptosis\u2010related <jats:italic>BAX<\/jats:italic> in PC\u20103, and decreased <jats:italic>AR<\/jats:italic>, <jats:italic>EGFR<\/jats:italic>, and <jats:italic>SNAI2<\/jats:italic> in 22Rv1 linked to proliferation and metastasis. These findings suggest potential for selegiline repurposing in both androgen\u2010sensitive and \u2010insensitive PAC therapy by promoting apoptosis and inhibiting cancer growth and survival signals, respectively.<\/jats:p>","DOI":"10.1002\/prp2.70173","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T12:02:18Z","timestamp":1757592138000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The <scp>MAO<\/scp>\u2010B Inhibitor Selegiline Reduces the Viability of Different Prostate Cancer Cell Lines and Enhances the Effects of Anti\u2010Androgen and Cytostatic Agents"],"prefix":"10.1002","volume":"13","author":[{"given":"Anita","family":"Steib","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School &amp; Centre for Neuroscience University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"E\u00f6tv\u00f6s Lor\u00e1nd Research Network Chronic Pain Research Group  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krisztina","family":"Poh\u00f3czky","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School &amp; Centre for Neuroscience University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"Department of Pharmacology, Faculty of Pharmacy University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"National Laboratory for Drug Research and Development  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School &amp; Centre for Neuroscience University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikt\u00f3ria","family":"Kormos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School &amp; Centre for Neuroscience University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"Kun","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School &amp; Centre for Neuroscience University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"National Laboratory for Drug Research and Development  Budapest Hungary"},{"name":"Hungarian Centre for Genomics and Bioinformatics, Szent\u00e1gothai Research Centre University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"K\u00e1lai","sequence":"additional","affiliation":[{"name":"Department of Organic and Pharmacological Chemistry, Faculty of Pharmacy University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Mangel","sequence":"additional","affiliation":[{"name":"Department of Oncotherapy, Medical School University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9ter","family":"M\u00e1tyus","sequence":"additional","affiliation":[{"name":"E\u2010Group ICT Software Zrt.  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