{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:34Z","timestamp":1786978414081,"version":"build-2736575974"},"reference-count":76,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp;amp; Perspec"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Jatropha gossypifolia<\/jats:italic>\n                    L., a member of the Euphorbiaceae family, has been traditionally used in the treatment of various ailments. However, its neuropharmacological, cytotoxic, and anthelmintic potentials have not been thoroughly investigated. The methanolic fruit extract of\n                    <jats:italic>J. gossypifolia<\/jats:italic>\n                    (JGF\u2010ME) was evaluated for anxiolytic activity using the Elevated Plus Maze (EPM), Hole Board Test (HBT), and Light\u2013Dark Box Test (LDT); antidepressant activity using the Forced Swimming Test (FST) and Tail Suspension Test (TST); and sedative effects through the Open Field and Hole Cross tests. Cytotoxicity was assessed via the Brine Shrimp Lethality Assay (BSLA), and anthelmintic activity was evaluated against\n                    <jats:italic>Pheretima posthuma<\/jats:italic>\n                    . GC\u2013MS was used for phytochemical screening, followed by molecular docking and ADME\/T analyses of the identified compounds. JGF\u2010ME exhibited a significant, dose\u2010dependent anxiolytic effect in EPM, HBT, and LDT. At 400\u2009mg\/kg, it significantly reduced immobility in FST and TST (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), indicating significant antidepressant activity. The extract also exhibited notable sedative effects, as evidenced by reduced locomotor activity in the Open Field and Hole Cross tests at doses of 200 and 400\u2009mg\/kg. In BSLA, JGF\u2010ME displayed moderate cytotoxicity (LC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u2009327.87\u2009\u03bcg\/mL) compared to colchicine (LC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200938.81\u2009\u03bcg\/mL). It also produced a dose\u2010dependent anthelmintic effect by paralyzing and killing\n                    <jats:italic>P. posthuma<\/jats:italic>\n                    . Molecular docking revealed high binding affinities of the identified compounds to selected human receptors, and in silico analysis suggested acceptable drug\u2010likeness; experimental validation is needed. The study confirms the neuropharmacological, cytotoxic, and anthelmintic potential of\n                    <jats:italic>J. gossypifolia<\/jats:italic>\n                    extract, supporting its traditional use and therapeutic promise.\n                  <\/jats:p>","DOI":"10.1002\/prp2.70185","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T15:08:51Z","timestamp":1761404931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comprehensive Evaluation of Methanolic Fruits Extract of\n                    <i>Jatropha gossypifolia<\/i>\n                    L.: Neuropharmacological, Cytotoxic, Anthelmintic,\n                    <scp>GC<\/scp>\n                    \u2013\n                    <scp>MS<\/scp>\n                    Profiling, and Molecular Docking Studies"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9733-3525","authenticated-orcid":false,"given":"Md.","family":"Arafat","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology University of Science and Technology Chittagong  Foy's Lake Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nusrat Jahan","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Daffodil International University  Birulia Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-899X","authenticated-orcid":false,"given":"Mahathir","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Department of Chemistry Chittagong University of Engineering &amp; Technology  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1926-6407","authenticated-orcid":false,"given":"Md. Jahirul Islam","family":"Mamun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6973-8289","authenticated-orcid":false,"given":"Jannatul Naima","family":"Meem","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9514-1601","authenticated-orcid":false,"given":"Md. Hossain","family":"Rasel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borhan","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0198-421X","authenticated-orcid":false,"given":"Md. Safayat Hossen","family":"Momen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences University of Chittagong  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1945-6598","authenticated-orcid":false,"given":"Md. 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