{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T03:59:46Z","timestamp":1780891186985,"version":"3.54.1"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["22JCYBJC00220"],"award-info":[{"award-number":["22JCYBJC00220"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["22JCYBJC00210"],"award-info":[{"award-number":["22JCYBJC00210"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Folate is an indispensable nutrient involved in key biological processes, including enzymatic reactions, DNA replication, metabolic regulation, and methylation. Studies indicate that folate promotes neuronal regeneration and repair in patients with spinal cord injury (SCI); however, its precise mechanisms remain unclear. We employed network pharmacology to identify potential targets of folate for SCI treatment; we conducted a clinical retrospective study, selecting 50 SCI patients and 50 non\u2010SCI control subjects from Tianjin Hospital between 2022 and 2025 to validate predictions by assessing serum folate levels. Network pharmacology identified 1402 folate\u2010related targets and 548 SCI\u2010related targets. Key targets included TNF\u2010\u03b1, CASP3, EGF, IL1\u03b2, and AKT1. Molecular docking revealed the highest binding affinity between folate and CASP3\/TNF\u2010\u03b1 (\u22128.5\u2009kcal\/mol). Clinical validation demonstrated statistically significant lower folate levels in SCI patients compared to normal levels in non\u2010SCI controls. A strong inverse correlation was observed between folate levels and injury level (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u2009\u22120.58,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Folate exerts neuroprotective effects by synergistically regulating neuronal apoptosis, inflammatory responses, and oxidative stress pathways. Clinical data reveal prevalent folate deficiency in SCI patients, and this nutritional deficit may exacerbate secondary injury cascades. We recommend incorporating folate supplementation into comprehensive SCI management protocols.\n                  <\/jats:p>","DOI":"10.1002\/prp2.70233","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T04:40:04Z","timestamp":1773376804000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Network Pharmacology Combined With Clinical Retrospective Analysis to Investigate the Potential Mechanisms and Clinical Significance of Folate in Treating Spinal Cord Injury"],"prefix":"10.1002","volume":"14","author":[{"given":"Xiaolei","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Tianjin University Tianjin Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6151-270X","authenticated-orcid":false,"given":"Jiajia","family":"Liang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine Institute of Sport, Exercise and Health, Tianjin University of Sport  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine Institute of Sport, Exercise and Health, Tianjin University of Sport  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine Institute of Sport, Exercise and Health, Tianjin University of Sport  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medical School Tianjin University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Tianjin University Tianjin Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwen","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine Institute of Sport, Exercise and Health, Tianjin University of Sport  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Tianjin University Tianjin Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13049\u2010017\u20100410\u20100"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100475\u20106"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13061782"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2017.12.008"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10143-018-1008-3"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1084101"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207533"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31823a07a2"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-D-23-00281"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/19390211.2024.2436842"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90133-A"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.5214\/ans.0972.7531.260205"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010019\u201001812\u20105"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/eep\/dvad002"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20174"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321\u2010020\u20100409\u20109"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2025.1568551"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26123731"},{"key":"e_1_2_14_20_1","volume-title":"Nutritional Anaemias: Report of a WHO Scientific Group [Meeting Held in Geneva From 13 to 17 March 1967]","author":"Anaemias W. S. G.","year":"1968"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11226685"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c130045"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102982.2009.01410.x"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2020.7413"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010020\u201003928\u2010y"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12040542"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914960"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032\u2010025\u201002652\u20101"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u2010695X.2007.00304.x"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201060761\u2010795\u20102_1"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/AGINGADV.AGINGADV\u2010D\u201024\u201000006"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7523"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181831f7e"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2004.21.1778"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6285099"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/174004"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2008.09.058"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2015.11.003"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.11.012"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5240127"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1068359"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758\u20102946\u20106\u201013"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1033919"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz382"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.00046"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv951"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku293"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4932153"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.15212\/bioi\u20102024\u20100066"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201044672\u20109"},{"key":"e_1_2_14_51_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S387893"},{"key":"e_1_2_14_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00702"},{"key":"e_1_2_14_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584\u2010023\u201001002\u20107"},{"key":"e_1_2_14_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20108\u2010426"},{"key":"e_1_2_14_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-5-p3"},{"key":"e_1_2_14_56_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S321877"},{"key":"e_1_2_14_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2023.100591"},{"key":"e_1_2_14_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj120"},{"key":"e_1_2_14_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e38851"},{"key":"e_1_2_14_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.1c00203"},{"key":"e_1_2_14_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1121"}],"container-title":["Pharmacology Research &amp; Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.70233","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prp2.70233","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prp2.70233","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T03:44:49Z","timestamp":1780890289000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/prp2.70233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,12]]},"references-count":60,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/prp2.70233"],"URL":"https:\/\/doi.org\/10.1002\/prp2.70233","archive":["Portico"],"relation":{},"ISSN":["2052-1707","2052-1707"],"issn-type":[{"value":"2052-1707","type":"print"},{"value":"2052-1707","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,12]]},"assertion":[{"value":"2025-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70233"}}