{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:41:54Z","timestamp":1792212114376,"version":"4.4.0"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    \u03b1\n                    <jats:sub>1<\/jats:sub>\n                    \u2010Adrenoceptors are an important drug target in benign prostatic hyperplasia (BPH) and have been intensively studied at the expression level, but their posttranslational regulation in the prostate is widely unknown. Here we examined modulation of prostate smooth muscle contractions by inhibitors for RabGTPases, which are involved in intracellular transport of internalized receptors and in vesicle function. Tissues were obtained from laser enucleation for BPH and from radical prostatectomy without prior BPH surgery. Contractions were induced in an organ bath, with and without the Rab27 inhibitor nexhinib20 or the Rab7 inhibitor CID1067700. Nexhinib20 reduced contractions induced by electric field stimulation (EFS) at 10\u2009\u03bcM in laser\u2010enucleated tissues (\n                    <jats:italic>E<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    79% [34\u2013125] of KCl\u2010induced contraction in controls, 40% [6\u201374] of KCl with nexhinib20,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0234), but not at 1\u2009\u03bcM or in prostatectomized tissues. Contractions by phenylephrine, methoxamine and noradrenaline were resistant to consistent modulation by nexhinib20 and CID1067700, both at 1\u2009\u03bcM and 10\u2009\u03bcM and in both types of prostate tissues. EFS\u2010induced contractions of laser\u2010enucleated and prostatectomized tissues remained unaltered with CID1067700. The lack of consistent effects of CID1067700 despite previously reported Rab7\u2010dependent regulation of \u03b1\n                    <jats:sub>1A<\/jats:sub>\n                    \u2010adrenoceptors questions the transferability of findings from receptor overexpression systems and biochemical small GTPase assays to endogenous receptor regulation in human tissues. Divergent responsiveness of neurogenic and agonist\u2010induced contractions to nexhinib20 and CID1067700 suggests Rab7\u2010independent regulation of \u03b1\n                    <jats:sub>1<\/jats:sub>\n                    \u2010adrenoceptor\u2010mediated contraction, but a possible presynaptic role of Rab27 in neurotransmission of human prostate smooth muscle.\n                  <\/jats:p>","DOI":"10.1002\/prp2.70331","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:26:21Z","timestamp":1792149981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adrenergic Contraction of Human Prostate Tissues Is Maintained Under the Rab\n                    <scp>GTPase<\/scp>\n                    Inhibitors Nexhinib20 and\n                    <scp>CID1067700<\/scp>"],"prefix":"10.1002","volume":"14","author":[{"given":"Luca","family":"Troffer","sequence":"first","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5905-1514","authenticated-orcid":false,"given":"Peibin","family":"Cen","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Brandstetter","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4659-2262","authenticated-orcid":false,"given":"Alexander","family":"Tamalunas","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian G.","family":"Stief","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1305-6727","authenticated-orcid":false,"given":"Martin","family":"Hennenberg","sequence":"additional","affiliation":[{"name":"Department of Urology LMU University Hospital, LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2023.04.008"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2023_678"},{"issue":"9","key":"e_1_2_13_4_1","first-page":"S3","article-title":"Pathophysiology, Epidemiology, and Natural History of Benign Prostatic Hyperplasia","volume":"6","author":"Lepor H.","year":"2004","journal-title":"Reviews in 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