{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:03:52Z","timestamp":1792181032298,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pharmacology Res &amp; Perspec"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cisplatin (CP), an anticancer drug, often causes kidney damage. However, the mechanism of CP\u2010induced acute kidney injury (AKI) is not completely understood. AKI was induced by intravenous injection (i.v.) of cisplatin at doses of 5, 8, and 10\u00a0mg\/kg. Anemoside B4 (B4) (20\u00a0mg\/kg, i.m.) and dexamethasone (DXM) (0.5\u00a0mg\/kg, i.v.) were used for AKI treatment. Biochemical indicators were assessed using an automatic biochemical analyzer, protein expression was analyzed by western blotting, and morphological changes in the kidney were examined by PAS staining. The serum creatinine (Cre) and blood urea nitrogen (BUN) levels did not change significantly in the first 2\u00a0days but abruptly increased on the third day after CP injection. The serum albumin (ALB) and total protein (TP) levels decreased in both a time\u2010 and dose\u2010dependent manner. The urine protein level increased, the clearing rate of Cre decreased distinctly, and morphologic changes appeared in a dose\u2010dependent manner. The protein expression of p53\/caspase\u20103, NLRP3, IL\u20106, and TNF\u2010\u03b1 was obviously upregulated on day 3; concurrently, nephrin and podocin were downregulated. The expression of LC3II and p62 was upregulated significantly as the CP dose increased. B4 and DXM obviously decreased the BUN and Cre levels after 3 or 5\u00a0days of treatment. AKI appeared distinctly in a time\u2010dependent manner at 2 to 5\u00a0days after the administration of 5\u00a0mg\/kg CP and in a dose\u2010dependent manner upon the administration of 5, 8, and 10\u00a0mg\/kg CP. The third day was a significant time point for renal deterioration, and treatment with B4 and DXM within the first 3\u00a0days provided significant protection against AKI.<\/jats:p>","DOI":"10.1002\/prp2.895","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T05:25:23Z","timestamp":1637904323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The abrupt pathological deterioration of cisplatin\u2010induced acute kidney injury: Emerging of a critical time point"],"prefix":"10.1002","volume":"9","author":[{"given":"Qin","family":"Gong","sequence":"first","affiliation":[{"name":"School of Pharmacy Jiangxi University of Traditional Chinese Medicine Nanchang China"},{"name":"Pharmacology Laboratory State Key Laboratory of Innovative Drugs and Efficient Energy\u2010saving Pharmaceutical Equipment Nanchang 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