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Significant effects on the mortality of <jats:italic>N. lugens<\/jats:italic> were observed, with 100% of the insects fed on the SFI1\/GNA fusion protein diet dead by day 7. The survival of the aphid <jats:italic>M. persicae<\/jats:italic> was also reduced when fed on the SFI1\/GNA fusion protein. After 14 days, only 49% of the aphids that were fed on the fusion protein were still alive compared with approximately 90% of the aphids fed on the control diet or on diet containing GNA only. The SFI1\/GNA fusion protein also slowed the development of <jats:italic>M. persicae<\/jats:italic>, and the reproductive capacity of the aphids fed on the SFI1\/GNA fusion protein was severely reduced. The ability of GNA to act as a carrier protein, and deliver the SFI1 neurotoxin to the haemolymph of <jats:italic>N. lugens<\/jats:italic>, following oral ingestion, was investigated. The successful delivery of intact SFI1\/GNA fusion protein to the haemolymph of these insects was shown by western blotting. Haemolymph taken from the insects that were fed on the fusion protein contained two GNA\u2010immunoreactive proteins of molecular weights corresponding to GNA and to the SFI1\/GNA fusion protein. \u00a9 2005 British Crown Copyright. Published for SCI by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ps.1119","type":"journal-article","created":{"date-parts":[[2005,10,4]],"date-time":"2005-10-04T05:46:26Z","timestamp":1128404786000},"page":"77-85","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach\u2010potato aphid, <i>Myzus persicae<\/i> (Hemiptera: Aphididae) and the rice brown planthopper, <i>Nilaparvata lugens<\/i> (Hemiptera: Delphacidae)"],"prefix":"10.1002","volume":"62","author":[{"given":"Rachel E","family":"Down","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine C","family":"Fitches","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan P","family":"Wiles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Corti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard A","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A","family":"Gatehouse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,10,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1998.0318"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1995.tb01956.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1996.tb00837.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1996.tb00836.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(96)00065-0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009616413886"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/j.1570-7458.1993.tb00699.x","article-title":"Antimetabolic effects of plant lectins and fungal enzymes on the nymphal stages of two important rice pests, Nilaparvata lugens and Nephotettix cincticeps","volume":"66","author":"Powell KS","year":"1993","journal-title":"Entomol Exp Appl"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1570-7458.1995.tb01909.x","article-title":"Antifeedant effects of plant lectins and an enzyme on the adult stage of the rice brown planthopper, Nilaparvata lugens","volume":"75","author":"Powell KS","year":"1995","journal-title":"Entomol Exp Appl"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1995.tb01910.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1998.00226.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(99)00143-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1570-7458.2001.00803.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(94)90141-4"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(63)90057-X"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00033-4"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(02)00222-6"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0962-1075.2004.00474.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(01)00068-3"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(98)00054-7"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(99)00121-3"},{"key":"e_1_2_6_22_2","unstructured":"RaemaekersRJM Expression of functional plant lectins in heterologous systems. 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