{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:30:26Z","timestamp":1784129426850,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,15]],"date-time":"2007-10-15T00:00:00Z","timestamp":1192406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Resistance gene analogues (RGAs) have been isolated from many crops and offer potential in breeding for disease resistance through marker\u2010assisted selection, either as closely linked or as perfect markers. Many R\u2010gene sequences contain kinase domains, and indeed kinase genes have been reported as being proximal to R\u2010genes, making kinase analogues an additionally promising target. The first step towards utilizing RGAs as markers for disease resistance is isolation and characterization of the sequences.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Sugarcane clone US01\u20101158 was identified as resistant to yellow leaf caused by the sugarcane yellow leaf virus (SCYLV) and moderately resistant to rust caused by <jats:italic>Puccinia melanocephala<\/jats:italic> Sydow &amp; Sydow. Degenerate primers that had previously proved useful for isolating RGAs and kinase analogues in wheat and soybean were used to amplify DNA from sugarcane (<jats:italic>Saccharum<\/jats:italic> spp.) clone US\u201001\u20101158. Sequences generated from 1512 positive clones were assembled into 134 contigs of between two and 105 sequences. Comparison of the contig consensuses with the NCBI sequence database using BLASTx showed that 20 had sequence homology to nuclear binding site and leucine rich repeat (NBS\u2010LRR) RGAs, and eight to kinase genes. Alignment of the deduced amino acid sequences with similar sequences from the NCBI database allowed the identification of several conserved domains. The alignment and resulting phenetic tree showed that many of the sequences had greater similarity to sequences from other species than to one another.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> The use of degenerate primers is a useful method for isolating novel sugarcane RGA and kinase gene analogues. Further studies are needed to evaluate the role of these genes in disease resistance. Published in 2007 by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ps.1469","type":"journal-article","created":{"date-parts":[[2007,10,15]],"date-time":"2007-10-15T07:16:35Z","timestamp":1192432595000},"page":"48-56","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Isolation of nucleotide binding site\u2010leucine rich repeat and kinase resistance gene analogues from sugarcane (<i>Saccharum<\/i> spp.)"],"prefix":"10.1002","volume":"64","author":[{"given":"Neil C","family":"Glynn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack C","family":"Comstock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushma G","family":"Sood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phat M","family":"Dang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose X","family":"Chaparro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,10,15]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"ComstockJCandGilbertRA Sugarcane yellow leaf disease (SS\u2010AGR\u2010256) inFlorida Sugarcane Handbook 1st edition 1991 revised 2005 ed. by Gilbert RA. 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