{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:21:47Z","timestamp":1790518907474,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T00:00:00Z","timestamp":1364169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n \n Resistance to carbendazim and other benzimidazole fungicides in\n Botrytis cinerea<\/jats:italic>\n and most other fungi is usually conferred by one or several allelic mutations in the\n \u03b2<\/jats:italic>\n \u2010tubulin. Carbendazim resistance in\n Fusarium graminearum<\/jats:italic>\n , however, differs from that in\n B. cinerea<\/jats:italic>\n and other fungi in that\n F. graminearum<\/jats:italic>\n has two\n \u03b2<\/jats:italic>\n \u2010tubulins (Fgtub1 and Fgtub2) rather than one, and the resistance is conferred by mutations in the\n \u03b2<\/jats:italic>\n 2<\/jats:sub>\n \u2010tubulin. In a previous study, the\n \u03b2<\/jats:italic>\n 1<\/jats:sub>\n \u2010tubulin of\n F. graminearum<\/jats:italic>\n (Fgtub1) was replaced with the\n \u03b2<\/jats:italic>\n \u2010tubulin of\n B. cinerea<\/jats:italic>\n conferring carbendazim resistance (\n BctubE198A<\/jats:styled-content>\n ). The transformants were sensitive to carbendazim\n <\/jats:bold>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n BctubE198A<\/jats:styled-content>\n was successfully transferred into the\n \u03b2<\/jats:italic>\n 2<\/jats:sub>\n \u2010tubulin locus of\n F. graminearum<\/jats:italic>\n (Fgtub2) via homologous replacement. The mutants were still sensitive to carbendazim. Furthermore, Fgtub2 of the mutant\n 20C1<\/jats:styled-content>\n (Fgtub1 had been replaced with\n BctubE198A<\/jats:styled-content>\n ) and Fgtub1 of the mutant\n 20D10<\/jats:styled-content>\n (Fgtub2 had been replaced with\n BctubE198A<\/jats:styled-content>\n ) were deleted. Surprisingly, the mutants were also sensitive to carbendazim. Meanwhile, the biological characteristics of all the mutants were determined\n <\/jats:bold>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n \n The\n B. cinerea \u03b2<\/jats:italic>\n \u2010tubulin (Bctub) could complement the function of the two\n F. graminearum \u03b2<\/jats:italic>\n \u2010tubulins even when both were deleted. Expression of the\n \u03b2<\/jats:italic>\n \u2010tubulin conferring carbendazim resistance differs between pathogenic fungi. \u00a9 2012 Society of Chemical Industry\n <\/jats:bold>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.3474","type":"journal-article","created":{"date-parts":[[2012,12,11]],"date-time":"2012-12-11T12:03:49Z","timestamp":1355227429000},"page":"582-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Functional analysis of the\n \u03b2<\/i>\n 2<\/sub>\n \u2010tubulin gene of\n Fusarium<\/i>\n graminearum<\/i>\n and the\n \u03b2<\/i>\n \u2010tubulin gene of\n Botrytis<\/i>\n cinerea<\/i>\n by homologous replacement"],"prefix":"10.1002","volume":"69","author":[{"given":"ShengMing","family":"Liu","sequence":"first","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Key Laboratory of Pesticide Nanjing Jiangsu 210095 China"},{"name":"College of Forestry Henan University of Science and Technology Luoyang Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YaBing","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Key Laboratory of Pesticide Nanjing Jiangsu 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChangYan","family":"Ge","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Key Laboratory of Pesticide Nanjing Jiangsu 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChangJun","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Key Laboratory of Pesticide Nanjing Jiangsu 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MingGuo","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education Key Laboratory of Pesticide Nanjing Jiangsu 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/PD-78-0760"},{"key":"e_1_2_6_3_1","unstructured":"ChenLF BaiGHandDesjardinsAE Recent Advances in Wheat Head Scab Research in China. 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